Chitosan scaffolds with BMP-6 loaded alginate microspheres for periodontal tissue engineering

The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The...

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Bibliographic Details
Published in:Journal of microencapsulation 2012-12, Vol.29 (8), p.770-780
Main Authors: Soran, Zeliha, Ayd n, R. Seda T l, Gümü derelio lu, Menem e
Format: Article
Language:English
Subjects:
alginate microspheres
Alginates - chemistry
Animals
Biological and medical sciences
BMP-6
Bone Morphogenetic Protein 6 - metabolism
Chitosan - chemistry
chitosan scaffold
Culture Media
General pharmacology
Glucuronic Acid - chemistry
Hexuronic Acids - chemistry
Medical sciences
mesenchymal stem cells
Mesenchymal Stromal Cells - metabolism
Microscopy, Electron, Scanning
Microspheres
periodontal tissue engineering
Periodontium - metabolism
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Rats
Tissue Engineering
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Summary:The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The addition of these microspheres to chitosan gels produced a novel scaffold in which not only the pore sizes and interconnectivity were preserved, but also a controlled release vehicle was generated. Loading capacity was adjusted as 50 ng or 100 ng BMP-6 for each scaffold and the controlled release behaviour of BMP-6 from chitosan scaffolds was observed during seven days. Cell culture studies were carried out with rat mesenchymal stem cells derived from bone marrow in three groups; chitosan scaffolds, chitosan scaffolds containing BMP-6-loaded alginate microspheres and chitosan scaffolds with free BMP-6 in culture medium. Results showed that controlled delivery of BMP-6 from alginate microspheres has a significant effect on osteogenic differentiation.
ISSN:0265-2048
1464-5246
DOI:10.3109/02652048.2012.686531