Molecular-Based Method To Distinguish the Sibling Species Hemigrapsus penicillatus and Hemigrapsus takanoi (Decapoda: Brachyura: Varunidae)

In this study, we developed a simple and rapid restriction fragment length polymorphism (RFLP) method to distinguish the sibling species Hemigrapsus penicillatus and H. takanoi based on interspecific base substitutions within the 16S rRNA region of mitochondrial DNA. Sequencing and alignment of part...

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Bibliographic Details
Published in:Journal of crustacean biology 2011-10, Vol.31 (4), p.577-581
Main Authors: Yamasaki, Izumi, Mingkid, Winda Mercedes, Strüssmann, Carlos Augusto, Doi, Wataru, Watanabe, Seiichi, Yokota, Masashi
Format: Article
Language:English
Subjects:
Biology
Brachyura
Crabs
Crustaceans
Decapoda
Fisheries science
Hemigrapsus
Hemigrapsus penicillatus
invasion
Invasive species
Mitochondrial DNA
mtDNA
Pigmentation
Polymerase chain reaction
RFLP
River ports
Sibling species
Special Section: Microsystematics of Hemigrapsus
Varunidae
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Summary:In this study, we developed a simple and rapid restriction fragment length polymorphism (RFLP) method to distinguish the sibling species Hemigrapsus penicillatus and H. takanoi based on interspecific base substitutions within the 16S rRNA region of mitochondrial DNA. Sequencing and alignment of partial sequences revealed two haplotypes for H. penicillatus and one for H. takanoi, and five species-specific base substitutions. Digestion of the PCR products using the restriction endonuclease Dde I produced a banding pattern that was consistent with species identification based on morphological and pigmentation patterns. The sequence for H. takanoi collected in Japan showed high homology with that reported for specimens collected in Europe. This method could be useful for identification of these two species by researchers without specific taxonomic knowledge.
ISSN:0278-0372
1937-240X
DOI:10.1651/10-3366.1