Osteoblast and osteoclast behavior in zebrafish cultured scales

Fish scale culture can be used as a model to test the effects of several molecules on bone metabolism by histological and biochemical methods, although solid cell biology data about the behavior of the scale cells in culture are needed if such a model is to be employed for pharmacological applicatio...

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Bibliographic Details
Published in:Cell and tissue research 2012-10, Vol.350 (1), p.69-75
Main Authors: Pasqualetti, Sara, Banfi, Giuseppe, Mariotti, Massimo
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Analysis
Animal Structures - cytology
Animal Structures - enzymology
Animals
Biomedical and Life Sciences
Biomedicine
Bones
Cell Nucleus - metabolism
Cell Shape
Cell Survival
Cells
Cells, Cultured
Cellular biology
Danio rerio
Fish
Freshwater
Human Genetics
Indoles - metabolism
Isoenzymes - metabolism
Metabolism
Molecular Medicine
Osteoblasts - cytology
Osteoblasts - metabolism
Osteoclasts - cytology
Osteoclasts - metabolism
Physiological aspects
Proteomics
Regular Article
Staining and Labeling
Tartrate-Resistant Acid Phosphatase
Temperature
Time Factors
Zebrafish - anatomy & histology
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Summary:Fish scale culture can be used as a model to test the effects of several molecules on bone metabolism by histological and biochemical methods, although solid cell biology data about the behavior of the scale cells in culture are needed if such a model is to be employed for pharmacological applications. In the present study, we cultured zebrafish scales at various temperatures and for various times and analyzed the behavior of the bone cells in terms of viability and activity. We demonstrated that the cultured scale cells maintained their usual distribution at 28°C until 72 h, after which time episquamal osteoblasts showed an obvious change in their cell organization followed by an increase in cell death. Osteoclast tartrate-resistant acid phosphatase and osteoblast alkaline phosphatase activities were maintained until 72 h but were reduced at 96 h as a consequence of the massive cell death. This scenario indicates that zebrafish scales cultured until 72 h can be considered as an innovative model of explanted organ culture to assay the ability of chemical compounds to modulate the metabolism of bone cells.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-012-1436-2