Insecticidal activity of Hypnum cupressiforme (Bryophyta) against Sitophilus granarius (Coleoptera: Curculionidae)

• Published in: Journal of stored products research 2012-10, Vol.51, p.6-10
• Main Authors: Abay, Gökhan, Karakoç, Ömer Cem, Tüfekçi, Ali Rıza, Koldaş, Serkan, Demirtas, Ibrahim
• Format: Article
• Language: English
• Subjects: Active fractions; adults; Agronomy. Soil science and plant productions; autumn; Biological and medical sciences; Bryophyta; Bryophytes; chromatography; Coleoptera; Contact toxicities; Control; Curculionidae; Fundamental and applied biological sciences. Psychology; gametophytes; General agronomy. Plant production; Granary weevils; Harvesting. Postharvest. Storage; Hypnum cupressiforme; insecticidal properties; Invertebrates; lethal concentration 50; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; Mosses; mosses and liverworts; national parks; Pests of stored products; Phytopathology. Animal pests. Plant and forest protection; plant anatomy; Protozoa. Invertebrates; Sitophilus granarius; temperature; Vegetative propagation. Sowing and planting. Harvesting
• ISSN: 0022-474X; 1879-1212
• DOI: 10.1016/j.jspr.2012.05.005

The moss Hypnum cupressiforme Hedw. is cosmopolitan throughout the world and is one of the most common moss species in Turkey. In the autumn of 2011, moss samples were collected from Şadıman Hill located in Ilgaz Mountain National Park in Turkey. After drying the collected samples at room temperature, the green gametophytes and brownish rhizoid parts of the plant were separated from other contents and ground up before extraction procedure. The contact toxicities of 14 column fractions and 11 flash fractions of H. cupressiforme were evaluated against granary weevil (Sitophilus granarius) adults. The fractions C-10 (70.62%) obtained from column chromatography and F-11 (64.48%) obtained from flash chromatography showed the highest activity. As a result of dose dependent studies carried out on C-10 and F-11, LC50 values were calculated as 44.8 μg/μl and 45.3 μg/μl, respectively. Two other fractions C-3 (29.9%) and F-2 (32.1%) were determined to have an active substance different from fractions C-10 and F-11. The LC50 values of fractions C-3 and F-2 were determined as 81.3 μg/μl and 124.6 μg/μl, respectively. ► The bioassay-guided fractionation of Hypnum cupressiforme was performed. ► The fractions were tested against Sitophilus granarius. ► The extract and its semi-purified fractions showed strong insecticidal activity. ► We determined the effective fractions for C-10 and F-11 with dose dependent studies. ► The results showed that some of the fractions could control S. granarius up to 24 h.