Loading…

Isolation and Characterization of Chitinase from Tomato Infected by Fusarium oxysporum f. sp. lycopersici

Chitinases are important component of plant defence in response to attack by pathogens. To identify specific chitinase, we constructed a cDNA library using total RNA from a genotype‐resistant tomato inoculated with conidia of isolates race 2 of Fusarium oxysporum f.sp. lycopersici (Fol). One chitina...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of phytopathology 2012-12, Vol.160 (11-12), p.741-744
Main Authors:	do Amaral, Daniel Oliveira Jordão, de Almeida, Clébia Maria Alves, Correia, Maria Tereza dos Santos, de Menezes Lima, Vera Lúcia, da Silva, Márcia Vanusa
Format:	Article
Language:	English
Subjects:	Chitinase Conidia double prime R protein Fusarium oxysporum Fusarium wilt Genomes Genotypes induced expression Infection Lycopersicon esculentum Pathogens Polymerase chain reaction PR proteins RNA Solanum lycopersicum Southern blotting
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c4400-46969012d51f7f3ad5d6e0207fd2bc3d9b2b7c844d3e4fd6ad75dae7a48784fb3
cites	cdi_FETCH-LOGICAL-c4400-46969012d51f7f3ad5d6e0207fd2bc3d9b2b7c844d3e4fd6ad75dae7a48784fb3
container_end_page	744
container_issue	11-12
container_start_page	741
container_title	Journal of phytopathology
container_volume	160
creator	do Amaral, Daniel Oliveira Jordão de Almeida, Clébia Maria Alves Correia, Maria Tereza dos Santos de Menezes Lima, Vera Lúcia da Silva, Márcia Vanusa
description	Chitinases are important component of plant defence in response to attack by pathogens. To identify specific chitinase, we constructed a cDNA library using total RNA from a genotype‐resistant tomato inoculated with conidia of isolates race 2 of Fusarium oxysporum f.sp. lycopersici (Fol). One chitinase (SolChi) clone was isolated and sequence analysis shows that the cDNA clone SolChi encodes an acidic isoform of class III chitinase. Southern blotting indicated that SolChi was present only once in the tomato genome. Real‐time quantitative RT‐PCR assay show that the expression of this gene is induced upon infection with Fol, and the accumulation of transcripts for this R protein was rapid in the resistant genotype during the first 24 h. A putative role for chitinase in tomato is defence against fungal pathogens.
doi_str_mv	10.1111/j.1439-0434.2012.01960.x
format	article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1171879437</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2800698071</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4400-46969012d51f7f3ad5d6e0207fd2bc3d9b2b7c844d3e4fd6ad75dae7a48784fb3</originalsourceid><addsrcrecordid>eNqNkVFr3SAYhqVs0LNu_0HYTW-SadSYXOyihPX0lNIV2q6XYqIyz5KYasJO-utrmtGL3qyC-KHP8_HJCwDEKMVxfdunmJIyQZTQNEM4SxEuc5QejsDm9eED2KCS4ATzgh2DTyHsEcoQQWgD7C64Vo7W9VD2Cla_pZfNqL19Wi-diXd2tL0MGhrvOnjnOjk6uOuNjqCC9QzPpyC9nTroDnMYnI-VSWEYUtjOjRu0D7axn8FHI9ugv_w7T8D9-Y-76iK5-rndVWdXSUMpQgnNy7yM_1AMG26IVEzlOk7LjcrqhqiyzmreFJQqoqlRuVScKam5pAUvqKnJCThd-w7ePU46jKKzodFtK3vtpiAw5rjgJSU8ol_foHs3-T5OFynM8ixu9j-KkZLRpVexUo13IXhtxOBtJ_0sMFo4LPZiCUQsgYglKfGSlDhE9fuq_rWtnt_ticubi6WKfrL6Noz68OpL_0fknHAmHq634qG6vWbb6pdg5BnazKhx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1115539547</pqid></control><display><type>article</type><title>Isolation and Characterization of Chitinase from Tomato Infected by Fusarium oxysporum f. sp. lycopersici</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>do Amaral, Daniel Oliveira Jordão ; de Almeida, Clébia Maria Alves ; Correia, Maria Tereza dos Santos ; de Menezes Lima, Vera Lúcia ; da Silva, Márcia Vanusa</creator><creatorcontrib>do Amaral, Daniel Oliveira Jordão ; de Almeida, Clébia Maria Alves ; Correia, Maria Tereza dos Santos ; de Menezes Lima, Vera Lúcia ; da Silva, Márcia Vanusa</creatorcontrib><description>Chitinases are important component of plant defence in response to attack by pathogens. To identify specific chitinase, we constructed a cDNA library using total RNA from a genotype‐resistant tomato inoculated with conidia of isolates race 2 of Fusarium oxysporum f.sp. lycopersici (Fol). One chitinase (SolChi) clone was isolated and sequence analysis shows that the cDNA clone SolChi encodes an acidic isoform of class III chitinase. Southern blotting indicated that SolChi was present only once in the tomato genome. Real‐time quantitative RT‐PCR assay show that the expression of this gene is induced upon infection with Fol, and the accumulation of transcripts for this R protein was rapid in the resistant genotype during the first 24 h. A putative role for chitinase in tomato is defence against fungal pathogens.</description><identifier>ISSN: 0931-1785</identifier><identifier>EISSN: 1439-0434</identifier><identifier>DOI: 10.1111/j.1439-0434.2012.01960.x</identifier><language>eng</language><publisher>Berlin: Blackwell Publishing Ltd</publisher><subject>Chitinase ; Conidia ; double prime R protein ; Fusarium oxysporum ; Fusarium wilt ; Genomes ; Genotypes ; induced expression ; Infection ; Lycopersicon esculentum ; Pathogens ; Polymerase chain reaction ; PR proteins ; RNA ; Solanum lycopersicum ; Southern blotting</subject><ispartof>Journal of phytopathology, 2012-12, Vol.160 (11-12), p.741-744</ispartof><rights>2012 Blackwell Verlag GmbH</rights><rights>Copyright © 2012 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4400-46969012d51f7f3ad5d6e0207fd2bc3d9b2b7c844d3e4fd6ad75dae7a48784fb3</citedby><cites>FETCH-LOGICAL-c4400-46969012d51f7f3ad5d6e0207fd2bc3d9b2b7c844d3e4fd6ad75dae7a48784fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>do Amaral, Daniel Oliveira Jordão</creatorcontrib><creatorcontrib>de Almeida, Clébia Maria Alves</creatorcontrib><creatorcontrib>Correia, Maria Tereza dos Santos</creatorcontrib><creatorcontrib>de Menezes Lima, Vera Lúcia</creatorcontrib><creatorcontrib>da Silva, Márcia Vanusa</creatorcontrib><title>Isolation and Characterization of Chitinase from Tomato Infected by Fusarium oxysporum f. sp. lycopersici</title><title>Journal of phytopathology</title><addtitle>J Phytopathol</addtitle><description>Chitinases are important component of plant defence in response to attack by pathogens. To identify specific chitinase, we constructed a cDNA library using total RNA from a genotype‐resistant tomato inoculated with conidia of isolates race 2 of Fusarium oxysporum f.sp. lycopersici (Fol). One chitinase (SolChi) clone was isolated and sequence analysis shows that the cDNA clone SolChi encodes an acidic isoform of class III chitinase. Southern blotting indicated that SolChi was present only once in the tomato genome. Real‐time quantitative RT‐PCR assay show that the expression of this gene is induced upon infection with Fol, and the accumulation of transcripts for this R protein was rapid in the resistant genotype during the first 24 h. A putative role for chitinase in tomato is defence against fungal pathogens.</description><subject>Chitinase</subject><subject>Conidia</subject><subject>double prime R protein</subject><subject>Fusarium oxysporum</subject><subject>Fusarium wilt</subject><subject>Genomes</subject><subject>Genotypes</subject><subject>induced expression</subject><subject>Infection</subject><subject>Lycopersicon esculentum</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>PR proteins</subject><subject>RNA</subject><subject>Solanum lycopersicum</subject><subject>Southern blotting</subject><issn>0931-1785</issn><issn>1439-0434</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkVFr3SAYhqVs0LNu_0HYTW-SadSYXOyihPX0lNIV2q6XYqIyz5KYasJO-utrmtGL3qyC-KHP8_HJCwDEKMVxfdunmJIyQZTQNEM4SxEuc5QejsDm9eED2KCS4ATzgh2DTyHsEcoQQWgD7C64Vo7W9VD2Cla_pZfNqL19Wi-diXd2tL0MGhrvOnjnOjk6uOuNjqCC9QzPpyC9nTroDnMYnI-VSWEYUtjOjRu0D7axn8FHI9ugv_w7T8D9-Y-76iK5-rndVWdXSUMpQgnNy7yM_1AMG26IVEzlOk7LjcrqhqiyzmreFJQqoqlRuVScKam5pAUvqKnJCThd-w7ePU46jKKzodFtK3vtpiAw5rjgJSU8ol_foHs3-T5OFynM8ixu9j-KkZLRpVexUo13IXhtxOBtJ_0sMFo4LPZiCUQsgYglKfGSlDhE9fuq_rWtnt_ticubi6WKfrL6Noz68OpL_0fknHAmHq634qG6vWbb6pdg5BnazKhx</recordid><startdate>201212</startdate><enddate>201212</enddate><creator>do Amaral, Daniel Oliveira Jordão</creator><creator>de Almeida, Clébia Maria Alves</creator><creator>Correia, Maria Tereza dos Santos</creator><creator>de Menezes Lima, Vera Lúcia</creator><creator>da Silva, Márcia Vanusa</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>201212</creationdate><title>Isolation and Characterization of Chitinase from Tomato Infected by Fusarium oxysporum f. sp. lycopersici</title><author>do Amaral, Daniel Oliveira Jordão ; de Almeida, Clébia Maria Alves ; Correia, Maria Tereza dos Santos ; de Menezes Lima, Vera Lúcia ; da Silva, Márcia Vanusa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4400-46969012d51f7f3ad5d6e0207fd2bc3d9b2b7c844d3e4fd6ad75dae7a48784fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Chitinase</topic><topic>Conidia</topic><topic>double prime R protein</topic><topic>Fusarium oxysporum</topic><topic>Fusarium wilt</topic><topic>Genomes</topic><topic>Genotypes</topic><topic>induced expression</topic><topic>Infection</topic><topic>Lycopersicon esculentum</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>PR proteins</topic><topic>RNA</topic><topic>Solanum lycopersicum</topic><topic>Southern blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>do Amaral, Daniel Oliveira Jordão</creatorcontrib><creatorcontrib>de Almeida, Clébia Maria Alves</creatorcontrib><creatorcontrib>Correia, Maria Tereza dos Santos</creatorcontrib><creatorcontrib>de Menezes Lima, Vera Lúcia</creatorcontrib><creatorcontrib>da Silva, Márcia Vanusa</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>do Amaral, Daniel Oliveira Jordão</au><au>de Almeida, Clébia Maria Alves</au><au>Correia, Maria Tereza dos Santos</au><au>de Menezes Lima, Vera Lúcia</au><au>da Silva, Márcia Vanusa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of Chitinase from Tomato Infected by Fusarium oxysporum f. sp. lycopersici</atitle><jtitle>Journal of phytopathology</jtitle><addtitle>J Phytopathol</addtitle><date>2012-12</date><risdate>2012</risdate><volume>160</volume><issue>11-12</issue><spage>741</spage><epage>744</epage><pages>741-744</pages><issn>0931-1785</issn><eissn>1439-0434</eissn><abstract>Chitinases are important component of plant defence in response to attack by pathogens. To identify specific chitinase, we constructed a cDNA library using total RNA from a genotype‐resistant tomato inoculated with conidia of isolates race 2 of Fusarium oxysporum f.sp. lycopersici (Fol). One chitinase (SolChi) clone was isolated and sequence analysis shows that the cDNA clone SolChi encodes an acidic isoform of class III chitinase. Southern blotting indicated that SolChi was present only once in the tomato genome. Real‐time quantitative RT‐PCR assay show that the expression of this gene is induced upon infection with Fol, and the accumulation of transcripts for this R protein was rapid in the resistant genotype during the first 24 h. A putative role for chitinase in tomato is defence against fungal pathogens.</abstract><cop>Berlin</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1439-0434.2012.01960.x</doi><tpages>4</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0931-1785
ispartof	Journal of phytopathology, 2012-12, Vol.160 (11-12), p.741-744
issn	0931-1785 1439-0434
language	eng
recordid	cdi_proquest_miscellaneous_1171879437
source	Wiley-Blackwell Read & Publish Collection
subjects	Chitinase Conidia double prime R protein Fusarium oxysporum Fusarium wilt Genomes Genotypes induced expression Infection Lycopersicon esculentum Pathogens Polymerase chain reaction PR proteins RNA Solanum lycopersicum Southern blotting
title	Isolation and Characterization of Chitinase from Tomato Infected by Fusarium oxysporum f. sp. lycopersici
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T08%3A52%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20Characterization%20of%20Chitinase%20from%20Tomato%20Infected%20by%20Fusarium%20oxysporum%20f.%20sp.%20lycopersici&rft.jtitle=Journal%20of%20phytopathology&rft.au=do%20Amaral,%20Daniel%20Oliveira%20Jord%C3%A3o&rft.date=2012-12&rft.volume=160&rft.issue=11-12&rft.spage=741&rft.epage=744&rft.pages=741-744&rft.issn=0931-1785&rft.eissn=1439-0434&rft_id=info:doi/10.1111/j.1439-0434.2012.01960.x&rft_dat=%3Cproquest_cross%3E2800698071%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4400-46969012d51f7f3ad5d6e0207fd2bc3d9b2b7c844d3e4fd6ad75dae7a48784fb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1115539547&rft_id=info:pmid/&rfr_iscdi=true