Photocleavable DNA Barcode–Antibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells

DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we descri...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Chemical Society 2012-11, Vol.134 (45), p.18499-18502
Main Authors: Agasti, Sarit S, Liong, Monty, Peterson, Vanessa M, Lee, Hakho, Weissleder, Ralph
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Antibodies - chemistry
Cell Line, Tumor
DNA Barcoding, Taxonomic
Fluorescent Dyes - chemistry
Humans
Mice
Photochemical Processes
Proteins - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we describe the development and validation of a photocleavable DNA barcode–antibody conjugate method for rapid, quantitative, and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification, and readout. As a proof of principle, we demonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja307689w