Marked Increase of CYP24A1 mRNA Level in Hepatocellular Carcinoma Cell Lines Following Vitamin D Administration

• Published in: Anticancer research 2012-11, Vol.32 (11), p.4791-4796
• Main Authors: HORVATH, Evelin, LAKATOS, Peter, BALLA, Bernadett, PAL KOSA, Janos, TOBIAS, Balint, JOZILAN, Hasan, BORKA, Katalin, CSABA HORVATH, Henrik, KOVALSZKY, Ilona, SZALAY, Ferenc
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Cell Line, Tumor; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression - drug effects; Humans; Immunohistochemistry; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Medical sciences; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Steroid Hydroxylases - biosynthesis; Steroid Hydroxylases - genetics; Tumors; Vitamin D - pharmacology; Vitamin D3 24-Hydroxylase
• ISSN: 0250-7005; 1791-7530

1,25-Dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) inhibits cell growth and induces apoptosis in numerous types of tumors. We aimed to examine the mRNA and protein expression of 1,25(OH)(2)D(3)-inactivating CYP24A1 and mRNA expression of the activating CYP27B1 enzymes, as well as that of vitamin D receptor (VDR), in hepatocellular carcinoma (HCC) cell cultures in response to 1,25(OH)(2)D(3) administration. Increasing amounts of 1,25(OH)(2)D(3) (0.256-10 nM) were added to cultures of HepG2, Huh-Neo, Hep3B, Huh5-15 human HCC cell lines and cells then incubated for various time periods (30 min-28 h). The mRNA expression was analyzed by real time reverse transcription-polymerase chain reaction (RT-PCR). CYP24A1 protein in HepG2 cells was detected by immuncytochemistry. CYP24A1 mRNA expression significantly (p