Differential uptake of MRI contrast agents indicates charge-selective blood-brain interface in the crayfish

This study provides a new perspective on the long-standing problem of the nature of the decapod crustacean blood-brain interface. Previous studies of crustacean blood-brain interface permeability have relied on invasive histological, immunohistochemical and electrophysiological techniques, indicatin...

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Bibliographic Details
Published in:Cell and tissue research 2012-08, Vol.349 (2), p.493-503
Main Authors: Otopalik, Adriane G, Shin, Jane, Beltz, Barbara S, Sandeman, David C, Kolodny, Nancy H
Format: Article
Language:English
Subjects:
Animals
Arthropoda
Astacoidea - anatomy & histology
Astacoidea - metabolism
Biomedical and Life Sciences
Biomedicine
Blood-brain barrier
Blood-Brain Barrier - anatomy & histology
Blood-Brain Barrier - metabolism
Brain - anatomy & histology
Brain - metabolism
Cambaridae
Capillary Permeability
Cherax destructor
Contrast Media - pharmacokinetics
crayfish
Crustaceans
Freshwater
Gadolinium DTPA - pharmacokinetics
Human Genetics
image analysis
Immunohistochemistry
Magnetic Resonance Imaging
manganese
Manganese - pharmacokinetics
Marine biology
Molecular Medicine
nervous system
NMR
Nuclear magnetic resonance
Permeability
Proteomics
Regular Article
sinuses
tracer techniques
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Summary:This study provides a new perspective on the long-standing problem of the nature of the decapod crustacean blood-brain interface. Previous studies of crustacean blood-brain interface permeability have relied on invasive histological, immunohistochemical and electrophysiological techniques, indicating a leaky non-selective blood-brain barrier. The present investigation involves the use of magnetic resonance imaging (MRI), a method for non-invasive longitudinal tracking of tracers in real-time. Differential uptake rates of two molecularly distinct MRI contrast agents, namely manganese (Mn(II)) and Magnevist® (Gd-DTPA), were observed and quantified in the crayfish, Cherax destructor. Contrast agents were injected into the pericardium and uptake was observed with longitudinal MRI for approximately 14.5 h. Mn(II) was taken up quickly into neural tissue (within 6.5 min), whereas Gd-DTPA was not taken up into neural tissue and was instead restricted to the intracerebral vasculature or excreted into nearby sinuses. Our results provide evidence for a charge-selective intracerebral blood-brain interface in the crustacean nervous system, a structural characteristic once considered too complex for a lower-order arthropod.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-012-1413-9