Atrazine and PCB 153 and their effects on the proteome of subcellular fractions of human MCF-7 cells

• Published in: Biochimica et biophysica acta 2012-06, Vol.1824 (6), p.833-841
• Main Authors: Lasserre, Jean-Paul, Fack, Fred, Serchi, Tommaso, Revets, Dominique, Planchon, Sébastien, Renaut, Jenny, Hoffmann, Lucien, Gutleb, Arno C., Muller, Claude P., Bohn, Torsten
• Format: Article
• Language: English
• Subjects: 2D-DiGE; Atrazine; Atrazine - pharmacology; cell membranes; cytosol; Cytosol - metabolism; dimethyl sulfoxide; Endocrine Disruptors - pharmacology; estradiol; Estradiol - pharmacology; Estradiol - physiology; exposure; HSP27 Heat-Shock Proteins - metabolism; Humans; MCF-7 Cells; MCF-7 human cell; Membrane Proteins - metabolism; metabolism; PCB 153; pollutants; polychlorinated biphenyls; Polychlorinated Biphenyls - pharmacology; proteins; proteome; Proteome - metabolism; Stathmin - metabolism; stress response; Subcellular fraction; Tacrolimus Binding Proteins - metabolism; Western blotting
• ISSN: 1570-9639; 0006-3002; 1878-1454
• DOI: 10.1016/j.bbapap.2012.03.014

Several man-made organic pollutants including polychlorinated biphenyls (PCBs) and several pesticides may exhibit endocrine disrupting (ED) properties. These ED molecules can be comparatively persistent in the environment, and have shown to perturb hormonal activity and several physiological functions. The objective of this investigation was to study the impact of PCB 153 and atrazine on human MCF-7 cells, and to search for marker proteins of their exposure. Cells were exposed to environmentally high but relevant concentrations of atrazine (200ppb), PCB 153 (500ppb), 17-β estradiol (positive control, 10nM) and DMSO (0.1%, negative control) for t=36h (n=3 replicates/exposure group). Proteins from cell membrane and cytosol were isolated, and studied by 2D-DiGE. Differentially regulated proteins were trypsin-digested and identified by MALDI-ToF-ToF and NCBInr database. A total of 36 differentially regulated proteins (>|1.5| fold change, P