Inhibition of Staphylococcus aureus adherence to Caco-2 cells by lactobacilli and cell surface properties that influence attachment

• Published in: Anaerobe 2012-10, Vol.18 (5), p.508-515
• Main Authors: Ren, Dayong, Li, Chang, Qin, Yanqing, Yin, Ronglan, Li, Xiao, Tian, Mingyao, Du, Shouwen, Guo, Huanhuan, Liu, Cunxia, Zhu, Na, Sun, Dandan, Li, Yi, Jin, Ningyi
• Format: Article
• Language: English
• Subjects: adhesion; Antibiosis; Bacterial Adhesion; Caco-2 Cells; Cell surface; Cell surface properties; Cell Wall - chemistry; digestive system diseases; Epithelial cells; Gastrointestinal tract diseases; heat; human cell lines; Humans; Hydrophobic and Hydrophilic Interactions; Hydrophobicity; Inhibition adhesion; intestinal mucosa; Intestine; Lactobacilli; Lactobacillus; Lactobacillus - physiology; Lactobacillus plantarum; Lactobacillus salivarius; Lithium chloride; Opportunist infection; pathogenesis; Pathogens; Staphylococcus aureus; Staphylococcus aureus - pathogenicity; Surface Properties
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2012.08.001

Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillus plantarum were investigated in vitro to examine their ability to competitively exclude S. aureus. Various factors involved in attachment, including bacterial status and cell concentration, growth phase, competition patterns, and surface-layer protein extracts, were also investigated. Live lactobacilli in the mid-log growth phase exhibited maximum inhibitory activity when lactobacilli were pre- or co-incubated with S. aureus. However, the inhibitory activity was significantly reduced when the lactobacilli were inactivated by heating or treated with LiCl. Furthermore, both lactobacilli possessed certain cell surface properties, such as hydrophobicity, autoaggregation, and coaggregation ability. L. salivarius and L. plantarum strongly inhibited S. aureus adherence to Caco-2 cells and their inhibition activity was significantly influenced by several factors that affect adhesion inhibition. ► The tested two lactobacilli inhibited Staphylococcus aureus adherence to Caco-2 cells. ► Many factors influenced this inhibition activity. ► The inhibitory activity largely depended on physicochemical properties of the cell surface of the lactobacilli.