Paenibacillus taohuashanense sp. nov., a nitrogen-fixing species isolated from rhizosphere soil of the root of Caragana kansuensis Pojark

• Published in: Antonie van Leeuwenhoek 2012-11, Vol.102 (4), p.735-741
• Main Authors: Xie, Jian-Bo, Zhang, Li-Hong, Zhou, Yu-Guang, Liu, Hong-Can, Chen, San-Feng
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Bacteria; Bacterial Typing Techniques; Bacteriology; Base Composition; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Caragana; Caragana - microbiology; Cluster Analysis; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Economic plant physiology; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Life Sciences; Medical Microbiology; Microbiology; Microscopy, Electron; Miscellaneous; Molecular Sequence Data; Nitrogen Fixation; Nucleic Acid Hybridization; Oxidoreductases - genetics; Paenibacillus; Paenibacillus - classification; Paenibacillus - genetics; Paenibacillus - isolation & purification; Paenibacillus - metabolism; Phylogenetics; Phylogeny; Plant Roots - microbiology; Plant Sciences; Review Paper; Rhizosphere; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Soil microorganisms; Soil Science & Conservation; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-012-9773-4

A nitrogen-fixing bacterium, designated strain gs65 T , was isolated from a rhizosphere soil sample of Caragana kansuensis Pojark . Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain gs65 T is a member of the genus Paenibacillus . High levels of 16S rRNA gene similarity were found between strain gs65 T and Paenibacillus boreali s DSM 13188 T (97.5 %), Paenibacillus odorifer ATCC BAA-93 T (97.3 %), Paenibacillus durus DSM 1735 T (97.0 %) and Paenibacillus sophorae DSM23020 T (96.9 %). Levels of 16S rRNA gene sequence similarity between strain gs65 T and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA–DNA relatedness between strain gs65 T and P. borealis DSM 13188 T , P. odorifer ATCC BAA-93 T (97.3 %), P. durus DSM 1735 T and P. sophorae DSM23020 T were 35.9, 38.0, 34.2 and 35.5 % respectively. The DNA G+C content of strain gs65 T was determined to be 51.6 mol%. The major fatty acids were found to be iso-C 14:0 , anteiso-C 15:0 and iso-C 16:0 . On the basis of its phenotypic characteristics and levels of DNA–DNA hybridization, strain gs65 T is considered to represent a novel species of the genus Paenibacillus , for which the name Paenibacillus taohuashanense sp. nov. is proposed. The type strain is gs65 T (=CGMCC 1.12175 T  = DSM 25809 T ).