Spreading dynamics of cellular aggregates confined to adhesive bands

We examine the spreading of cellular aggregates deposited on adhesive striated glass surfaces consisting of 100μm large bands alternatively coated with fibronectin and with PolyEthyleneGlycol-Poly-L-lysine (PEG-PLL). The aggregates spread confined to the adhesive fibronectin bands. A front of cells...

Full description

Saved in:
Bibliographic Details
Published in:The European physical journal. E, Soft matter and biological physics Soft matter and biological physics, 2012-11, Vol.35 (11), p.116-116, Article 116
Main Authors: Douezan, Stéphane, Brochard-Wyart, Françoise
Format: Article
Language:English
Subjects:
Adhesives - chemistry
Adhesives - pharmacology
Animals
Biological and Medical Physics
Biological and medical sciences
Biophysics
Cell Aggregation - drug effects
Cell Line, Tumor
Complex Fluids and Microfluidics
Complex Systems
Fibronectins - chemistry
Fundamental and applied biological sciences. Psychology
Glass - chemistry
Mice
Nanotechnology
Physics
Physics and Astronomy
Polyethylene Glycols - chemistry
Polylysine - chemistry
Polymer Sciences
Regular Article
Soft and Granular Matter
Surface Properties
Surfaces and Interfaces
Thin Films
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We examine the spreading of cellular aggregates deposited on adhesive striated glass surfaces consisting of 100μm large bands alternatively coated with fibronectin and with PolyEthyleneGlycol-Poly-L-lysine (PEG-PLL). The aggregates spread confined to the adhesive fibronectin bands. A front of cells expands from the aggregate at constant velocity. In comparison, the radial spreading of an aggregate on the uniform fibronectin coated glass surface obeys a diffusive law. We develop a common theoretical model in agreement with our experimental observations to describe the apparently disparate spreading kinetics of cellular aggregates. These results demonstrate the dominant role of the permeation in the expansion of the precursor film of cells around the aggregate. Graphical abstract
ISSN:1292-8941
1292-895X
DOI:10.1140/epje/i2012-12116-8