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Spreading dynamics of cellular aggregates confined to adhesive bands
We examine the spreading of cellular aggregates deposited on adhesive striated glass surfaces consisting of 100μm large bands alternatively coated with fibronectin and with PolyEthyleneGlycol-Poly-L-lysine (PEG-PLL). The aggregates spread confined to the adhesive fibronectin bands. A front of cells...
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Published in: | The European physical journal. E, Soft matter and biological physics Soft matter and biological physics, 2012-11, Vol.35 (11), p.116-116, Article 116 |
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container_issue | 11 |
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container_title | The European physical journal. E, Soft matter and biological physics |
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creator | Douezan, Stéphane Brochard-Wyart, Françoise |
description | We examine the spreading of cellular aggregates deposited on adhesive striated glass surfaces consisting of 100μm large bands alternatively coated with fibronectin and with PolyEthyleneGlycol-Poly-L-lysine (PEG-PLL). The aggregates spread confined to the adhesive fibronectin bands. A front of cells expands from the aggregate at constant velocity. In comparison, the radial spreading of an aggregate on the uniform fibronectin coated glass surface obeys a diffusive law. We develop a common theoretical model in agreement with our experimental observations to describe the apparently disparate spreading kinetics of cellular aggregates. These results demonstrate the dominant role of the permeation in the expansion of the precursor film of cells around the aggregate.
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doi_str_mv | 10.1140/epje/i2012-12116-8 |
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Psychology</subject><subject>Glass - chemistry</subject><subject>Mice</subject><subject>Nanotechnology</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polylysine - chemistry</subject><subject>Polymer Sciences</subject><subject>Regular Article</subject><subject>Soft and Granular Matter</subject><subject>Surface Properties</subject><subject>Surfaces and Interfaces</subject><subject>Thin Films</subject><issn>1292-8941</issn><issn>1292-895X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAURYMozjj6B1xIN4KbOnlp06ZLGT9BcKGCu5BJXmqHNq3JVJh_b-fDcSdZJJBz73scQs6BXgOkdIrdAqcVo8BiYABZLA7IGFjBYlHwj8P9O4UROQlhQSkdYskxGbEEMppn6ZjcvnYelalcGZmVU02lQ9TaSGNd97XykSpLj6VaYoh062zl0ETLNlLmE0P1jdFcORNOyZFVdcCz3T0h7_d3b7PH-Pnl4Wl28xzrNE2WMSidc23QpMJQyueCYQqaMixgnlmaGYtc54IXzIjCWiYocBAWC6oUWponE3K17e18-9VjWMqmCutVlcO2DxIgF1nBOYcBZVtU-zYEj1Z2vmqUX0mgcm1Pru3JjT25sSfFELrY9ffzBs0-8qtrAC53gApa1dYrp6vwx2W5oMMZuGTLheHLlejlou29G9z8N_4HEh-JtA</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Douezan, Stéphane</creator><creator>Brochard-Wyart, Françoise</creator><general>Springer Berlin Heidelberg</general><general>EDP Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121101</creationdate><title>Spreading dynamics of cellular aggregates confined to adhesive bands</title><author>Douezan, Stéphane ; Brochard-Wyart, Françoise</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-1ac75cded48d005b82e41c02e91b6f06dfe5c78592d89ff2801518fe90aaef073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adhesives - chemistry</topic><topic>Adhesives - pharmacology</topic><topic>Animals</topic><topic>Biological and Medical Physics</topic><topic>Biological and medical sciences</topic><topic>Biophysics</topic><topic>Cell Aggregation - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Complex Fluids and Microfluidics</topic><topic>Complex Systems</topic><topic>Fibronectins - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glass - chemistry</topic><topic>Mice</topic><topic>Nanotechnology</topic><topic>Physics</topic><topic>Physics and Astronomy</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polylysine - chemistry</topic><topic>Polymer Sciences</topic><topic>Regular Article</topic><topic>Soft and Granular Matter</topic><topic>Surface Properties</topic><topic>Surfaces and Interfaces</topic><topic>Thin Films</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Douezan, Stéphane</creatorcontrib><creatorcontrib>Brochard-Wyart, Françoise</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European physical journal. 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The aggregates spread confined to the adhesive fibronectin bands. A front of cells expands from the aggregate at constant velocity. In comparison, the radial spreading of an aggregate on the uniform fibronectin coated glass surface obeys a diffusive law. We develop a common theoretical model in agreement with our experimental observations to describe the apparently disparate spreading kinetics of cellular aggregates. These results demonstrate the dominant role of the permeation in the expansion of the precursor film of cells around the aggregate.
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subjects | Adhesives - chemistry Adhesives - pharmacology Animals Biological and Medical Physics Biological and medical sciences Biophysics Cell Aggregation - drug effects Cell Line, Tumor Complex Fluids and Microfluidics Complex Systems Fibronectins - chemistry Fundamental and applied biological sciences. Psychology Glass - chemistry Mice Nanotechnology Physics Physics and Astronomy Polyethylene Glycols - chemistry Polylysine - chemistry Polymer Sciences Regular Article Soft and Granular Matter Surface Properties Surfaces and Interfaces Thin Films |
title | Spreading dynamics of cellular aggregates confined to adhesive bands |
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