Two-photon microscopy of the mouse cochlea in situ for cellular diagnosis

Sensorineural hearing loss is the most common type of hearing loss worldwide, yet the underlying cause is typically unknown because the inner ear cannot be biopsied today without destroying hearing, and intracochlear cells have not been imaged with resolution sufficient to establish diagnosis. Intra...

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Bibliographic Details
Published in:Journal of biomedical optics 2013-03, Vol.18 (3), p.031104-031104
Main Authors: Yang, Xin, Pu, Ye, Hsieh, Chia-Lung, Ong, Cheng Ai, Psaltis, Demetri, Stankovic, Konstantina M
Format: Article
Language:English
Subjects:
Animals
Casing (process)
Cellular
Cochlea
Diagnosis
Excitation
Hair Cells, Auditory - pathology
Hearing
Hearing Loss, Sensorineural - diagnosis
Hearing Loss, Sensorineural - pathology
Image Processing, Computer-Assisted - methods
Imaging
Male
Mice
Microscopy, Fluorescence, Multiphoton - methods
Organ of Corti - pathology
Sensory Receptor Cells - pathology
Windows (intervals)
Citations: Items that cite this one
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Description
Summary:Sensorineural hearing loss is the most common type of hearing loss worldwide, yet the underlying cause is typically unknown because the inner ear cannot be biopsied today without destroying hearing, and intracochlear cells have not been imaged with resolution sufficient to establish diagnosis. Intracochlear imaging has been technologically challenging because of the cochlea's small size and encasement in bone. We report, for the first time, imaging of the mouse cochlea in situ without exogenous dyes, through a membranous round window, using a near-infrared femtosecond laser as the excitation and endogenous two-photon excitation fluorescence (TPEF) and second harmonic generation as the contrast mechanisms. We find that TPEF exhibits strong contrast allowing cellular, and even subcellular resolution, and detection of specific, noise-induced pathologic changes. Our results demonstrate that the round window provides a useful access to the cochlea through the middle ear, and they motivate future development of a new and efficient diagnostic tool based on two-photon micro-endoscopy.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.18.3.031104