Isolation, characterization and phylogenetic analysis of halophilic archaea from a salt mine in central Anatolia (Turkey)

The haloarchaeal diversity of a salt mine, a natural cave in central Anatolia, was investigated using convential microbiological and molecular biology methods. Eight halophilic archaeal isolates selected based on their colony morphology and whole cell protein profiles were taxonomically classified o...

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Bibliographic Details
Published in:Polish journal of microbiology 2012, Vol.61 (2), p.111-117
Main Authors: Yildiz, Evrim, Ozcan, Birgul, Caliskan, Mahmut
Format: Article
Language:English
Subjects:
Archaea
DNA, Ribosomal - genetics
Halobacteriales - classification
Halobacteriales - genetics
Halobacteriales - isolation & purification
Halobacteriales - metabolism
Halophilic archaea
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S - genetics
Salt mines
Sodium Chloride - metabolism
Turkey
Water Microbiology
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Summary:The haloarchaeal diversity of a salt mine, a natural cave in central Anatolia, was investigated using convential microbiological and molecular biology methods. Eight halophilic archaeal isolates selected based on their colony morphology and whole cell protein profiles were taxonomically classified on the basis of their morphological, physiological, biochemical properties, polar lipid and protein profiles and 16S rDNA sequences. From the 16S rDNA sequences comparisons it was established that the isolates CH2, CH3 and CHC resembled Halorubrum saccharovorum by 98.8%, 98.9% and 99.5%, respectively. There was a 99.7% similarity between the isolate CH11 and Halobacterium noricense and 99.2% between the isolate CHA1 and Haloarcula argentinensis. The isolate CH8K and CH8B revealed a similarity rate of 99.8% and 99.3% to Halococcus dombrowskii, respectively. It was concluded that the isolates named CH2, CH3 and CHC were clustered in the genus Halorubrum and that CHA1 and CH7 in the genus Haloarcula, CH8K and CH8B in the genus Halococcus and CH11 in the genus Halobacterium.
ISSN:1733-1331
2544-4646
DOI:10.33073/pjm-2012-014