Cell cycle analysis of the CD133(+) and CD133(-) cells isolated from human colorectal cancer

The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary human colorectal tumors. After mechanical and enzymatic dissociation of the tumor sam...

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Bibliographic Details
Published in:Journal of cancer research and therapeutics 2012-07, Vol.8 (3), p.399-403
Main Authors: Gharagozloo, Marjan, Mirzaei, Hamid R, Bagherpour, Bahram, Rezaei, Abbas, Kalantari, Hamid, Sanei, Mohammad H, Hosseini, Mohsen, Mohajeri, Gholamreza, Tabatabai, Abbas, Hashemi, Mozaffar
Format: Article
Language:English
Subjects:
AC133 Antigen
Aged
Analysis
Antibiotics
Antigens, CD - metabolism
Biomarkers, Tumor - metabolism
Cancer therapies
Cell Cycle
Colon
Colorectal cancer
Colorectal Neoplasms - drug therapy
Colorectal Neoplasms - pathology
Confidence intervals
Diagnosis
Drug Resistance, Neoplasm
Female
Flow cytometry
Genetic aspects
Glycoproteins - metabolism
Health aspects
Humans
Liver Neoplasms - drug therapy
Liver Neoplasms - pathology
Liver Neoplasms - secondary
Magnetic fields
Male
Middle Aged
Neoplastic Stem Cells
Patients
Peptides - metabolism
Population
Stem cells
Studies
Tumor markers
Tumors
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Summary:The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary human colorectal tumors. After mechanical and enzymatic dissociation of the tumor samples, CD133(+) and CD133(-) subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133(+) and CD133(-) cells isolated from primary and liver metastatic cancer cells. The results indicated that CD133(+) cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133(-) cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133(-) cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase. The present study provides evidence that CD133(+) cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-therapy patients.
ISSN:0973-1482
1998-4138
DOI:10.4103/0973-1482.103520