Valproic acid induces the glutamate transporter excitatory amino acid transporter-3 in human oligodendroglioma cells

• Published in: Neuroscience 2012-12, Vol.227, p.260-270
• Main Authors: Bianchi, M.G, Franchi-Gazzola, R, Reia, L, Allegri, M, Uggeri, J, Chiu, M, Sala, R, Bussolati, O
• Format: Article
• Language: English
• Subjects: 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase - metabolism; Anticonvulsants - pharmacology; Anticonvulsants. Antiepileptics. Antiparkinson agents; Aspartic Acid - metabolism; Aspartic Acid - pharmacology; Biological and medical sciences; Cell Line, Tumor; EAAT3; Enzyme Inhibitors - pharmacology; Excitatory Amino Acid Transporter 3 - genetics; Excitatory Amino Acid Transporter 3 - metabolism; Gene Expression Regulation, Neoplastic - drug effects; Glial Fibrillary Acidic Protein - metabolism; glutamate transport; Humans; Medical sciences; Neurology; Neuropharmacology; oligodendrocyte precursor cells; oligodendroglioma; Oligodendroglioma - pathology; Pharmacology. Drug treatments; Phorbol 12,13-Dibutyrate - pharmacology; Protein Transport - drug effects; Receptor, Platelet-Derived Growth Factor alpha - genetics; Receptor, Platelet-Derived Growth Factor alpha - metabolism; RNA, Messenger - metabolism; Signal Transduction - drug effects; Time Factors; trichostatin A; Tritium - metabolism; Tubulin - metabolism; valproic acid; Valproic Acid - pharmacology
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2012.09.055

Graphical abstract Highlights ► Human oligodendroglioma Hs683 cells have no EAAT-dependent amino acid transport. ► Valproic acid (VPA, 1 mM) induces high-affinity aspartate transport in Hs683 cells. ► Transport stimulation is due to increased SLC1A1 mRNA and EAAT3 protein. ► VPA treatment also induces the early pre-oligodendrocytic marker PDGFRA . ► Trichostatin A mimics VPA effects, pointing to the epigenetic regulation of SLC1A1.