Effects of TNF inhibitor on innate inflammatory and Th17 cytokines in stimulated whole blood from rheumatoid arthritis patients

Background Recent studies point to important roles for IL-17 and Th17 cells in sustaining chronic inflammation and articular destruction in rheumatoid arthritis (RA). We investigated the effects of TNF inhibitor on innate inflammatory and Th17 cytokines production by ex vivo lipopolysaccharide (LPS)...

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Bibliographic Details
Published in:Inflammopharmacology 2012-12, Vol.20 (6), p.323-330
Main Authors: Zivojinovic, Sladjana, Pejnovic, Nada, Sefik-Bukilica, Mirjana, Kovacevic, Ljiljana, Soldatovic, Ivan, Bugarski, Diana, Mojsilovic, Slavko, Damjanov, Nemanja
Format: Article
Language:English
Subjects:
Adult
Aged
Allergology
Antirheumatic Agents - therapeutic use
Arthritis, Rheumatoid - blood
Arthritis, Rheumatoid - drug therapy
Arthritis, Rheumatoid - immunology
Autoantibodies - immunology
Biomedical and Life Sciences
Biomedicine
Cytokines - immunology
Dermatology
Etanercept
Female
Follow-Up Studies
Gastroenterology
Humans
Immunity, Innate - drug effects
Immunity, Innate - immunology
Immunoglobulin G - therapeutic use
Immunology
Interleukins - immunology
Lipopolysaccharides - immunology
Lipopolysaccharides - pharmacology
Male
Middle Aged
Pharmacology/Toxicology
Receptors, Tumor Necrosis Factor - therapeutic use
Research Article
Rheumatology
Th17 Cells - drug effects
Th17 Cells - immunology
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - immunology
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Summary:Background Recent studies point to important roles for IL-17 and Th17 cells in sustaining chronic inflammation and articular destruction in rheumatoid arthritis (RA). We investigated the effects of TNF inhibitor on innate inflammatory and Th17 cytokines production by ex vivo lipopolysaccharide (LPS)-stimulated whole blood in patients with RA and the associations of cytokine levels in whole blood cultures with autoantibodies and markers of disease activity. Materials and methods Whole blood cultures from 18 healthy volunteers and 19 RA patients on etanercept therapy were stimulated with LPS and the production of IL-6, TNF-α, IL-23, IL-17A and IL-21 was measured by ELISA. Results After stimulation with LPS, the interleukin (IL)-17A ( p  = 0.020) and IL-21 ( p  = 0.0001) secretions were significantly higher in patients with RA than in controls, while the TNF-α ( p  = 0.002) was significantly lower at baseline. Etanercept significantly decreased IL-21 production ( p  = 0.007), while IL-6 production ( p  = 0.005) significantly increased after 6 months of therapy. IL-21 significantly correlated with RF ( r  = 0.917, p  
ISSN:0925-4692
1568-5608
DOI:10.1007/s10787-012-0143-7