The effect of vascular endothelial growth factor on aggrecan and type II collagen expression in rat articular chondrocytes

• Published in: Rheumatology international 2012-11, Vol.32 (11), p.3359-3364
• Main Authors: Chen, Xuan-yin, Hao, Ya-rong, Wang, Zhe, Zhou, Jian-lin, Jia, Qi-xue, Qiu, Bo
• Format: Article
• Language: English
• Subjects: Aggrecans - genetics; Aggrecans - metabolism; Animals; Cartilage, Articular - cytology; Cartilage, Articular - drug effects; Cartilage, Articular - metabolism; Cells, Cultured; Chondrocytes - cytology; Chondrocytes - drug effects; Chondrocytes - metabolism; Collagen Type II - genetics; Collagen Type II - metabolism; Knee Joint - cytology; Knee Joint - drug effects; Knee Joint - metabolism; Medicine; Medicine & Public Health; Original Article; Rats; Rats, Sprague-Dawley; Rheumatology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Vascular Endothelial Growth Factor A - pharmacology
• ISSN: 0172-8172; 1437-160X
• DOI: 10.1007/s00296-011-2178-2

The expression of vascular endothelial growth factor (VEGF) directly correlates with the Mankin score and the degree of cartilage destruction. The biological activity of VEGF on articular cartilage remains unknown, so this study was performed to investigate the effect of VEGF on aggrecan and type II collagen expression in vitro. We carried out this study at the Center Laboratory of Renmin Hospital at Wuhan University. Rat articular chondrocytes were cultured in a monolayer. Then, the experiment was divided into 4 groups: group A (control group), without any disposal; group B, treated with 10 ng/ml VEGF; group C, treated with 10 ng/ml IL-1β; and group D, treated with 10 ng/ml VEGF + 10 ng/ml IL-1β. After 48 h, messenger RNA (mRNA) expression of aggrecan and type II collagen was evaluated by real-time polymerase chain reaction (real-Time PCR), and protein expression of aggrecan and type II collagen was detected by Western blotting. VEGF was found to significantly inhibit the expression of aggrecan and type II collagen at the gene and protein levels. These findings suggest that VEGF may result in degeneration of articular cartilage by inhibiting the synthesis and expression of aggrecan and type II collagen.