Lipoproteins of Actinomyces viscosus induce inflammatory responses through TLR2 in human gingival epithelial cells and macrophages

Actinomyces viscosus has been suggested to be associated with periodontal disease. However, the pathogenicity of this bacterium is not known. In this study, we examined inflammation-inducing activity by A. viscosus. Whole cells and a lipophilic fraction of A. viscosus ATCC19246 induced production of...

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Bibliographic Details
Published in:Microbes and infection 2012-09, Vol.14 (11), p.916-921
Main Authors: Shimada, Eri, Kataoka, Hideo, Miyazawa, Yasushi, Yamamoto, Matsuo, Igarashi, Takeshi
Format: Article
Language:English
Subjects:
Actinomyces viscosus
Actinomyces viscosus - chemistry
Actinomyces viscosus - immunology
Actinomycosis - immunology
Actinomycosis - microbiology
Analysis of Variance
Antibodies
Bacterial Proteins - immunology
Bacteriology
Biological and medical sciences
Cytokines - immunology
Epithelial cells
Epithelial Cells - cytology
Epithelial Cells - immunology
Fundamental and applied biological sciences. Psychology
Gingiva
Gingiva - cytology
Gingiva - immunology
Gingival Diseases - immunology
Gingival Diseases - microbiology
HEK293 Cells
Host-Pathogen Interactions
Humans
Inflammation
Inflammation - immunology
Interleukin 8
Lipophilic
Lipoprotein
Lipoprotein lipase
Lipoproteins
Lipoproteins - immunology
Macrophages
Macrophages - cytology
Macrophages - immunology
Microbiology
Miscellaneous
Pathogenicity
Periodontal diseases
Periodontitis
TLR2 protein
Toll-like receptor 2
Toll-Like Receptor 2 - immunology
Toll-like receptors
Tumor necrosis factor- alpha
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Summary:Actinomyces viscosus has been suggested to be associated with periodontal disease. However, the pathogenicity of this bacterium is not known. In this study, we examined inflammation-inducing activity by A. viscosus. Whole cells and a lipophilic fraction of A. viscosus ATCC19246 induced production of interleukin-8 and tumor necrosis factor alpha from both human oral epithelial cells and human monocytoid cells. This cytokine production was blocked by lipoprotein lipase treatment of the lipophilic fraction. In addition, anti-Toll-like receptor 2 antibody blocked the cytokine production. These results suggest that lipoprotein of A. viscosus triggers inflammatory responses in periodontitis by activation of Toll-like receptor 2.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2012.04.015