Isolation of novel microsatellite loci in the black goby Gobius niger and cross-amplification in other gobiid species (Perciformes, Gobiidae)

Twelve microsatellite loci were isolated from and characterized for the black goby Gobius niger. These loci were tested on a total of 48 individuals from two geographically distant locations (Orbetello and Cattolica) and the number of alleles ranged from two to 18, with expected (He) and observed (H...

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Bibliographic Details
Published in:Journal of fish biology 2012-11, Vol.81 (6), p.2044-2052
Main Authors: Ruggeri, P., Splendiani, A., Giovannotti, M., Nisi Cerioni, P., Caputo, V.
Format: Article
Language:English
Subjects:
Alleles
Amplified Fragment Length Polymorphism Analysis
Animals
Aphia
DNA Primers
FIASCO
genetic marker
Gobiidae
Gobius
Gobius niger
Heterozygote
Lesueurigobius
Microsatellite Repeats
Perciformes
Perciformes - genetics
population genetic
SSRS
Zosterisessor
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Summary:Twelve microsatellite loci were isolated from and characterized for the black goby Gobius niger. These loci were tested on a total of 48 individuals from two geographically distant locations (Orbetello and Cattolica) and the number of alleles ranged from two to 18, with expected (He) and observed (Ho) heterozygosities ranging from 0·042 to 0·941 and from 0·042 to 0·917, respectively. The loci described were used to cross‐amplify other gobiid species belonging to Gobius, Zosterisessor, Lesueurigobius and Aphia.
ISSN:0022-1112
1095-8649
DOI:10.1111/j.1095-8649.2012.03444.x