Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep

• Published in: Molecular biology reports 2012-10, Vol.39 (10), p.9801-9807
• Main Authors: He, J. N., Zhang, B. Y., Chu, M. X., Wang, P. Q., Feng, T., Cao, G. L., Di, R., Fang, L., Huang, D. W., Tang, Q. Q., Li, N.
• Format: Article
• Language: English
• Subjects: 5' Flanking Region; Animal Anatomy; Animal Biochemistry; Animal reproduction; Animals; Antibodies; Base Sequence; Biomedical and Life Sciences; Breeding; Exons; Female; Gene Frequency; Gene polymorphism; Genetic Association Studies; Genotype; Genotype & phenotype; Histology; Igf1 gene; Insulin-Like Growth Factor I - genetics; Insulin-like growth factors; Life Sciences; Litter Size - genetics; marker-assisted selection; Microsatellite Repeats; Microsatellites; Molecular Sequence Data; Morphology; Mutation; Polymerase chain reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Population genetics; Regulatory sequences; Restriction fragment length polymorphism; Sequence Analysis, DNA; Sheep; Sheep - genetics
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-012-1846-y

The insulin-like growth factor 1 ( IGF1 ) gene was studied as a candidate gene for high prolificacy in sheep. Polymorphisms of 5′ regulatory region and all four exons of IGF1 gene were detected in Small Tail Han ( n  = 277), Hu ( n  = 58), Texel ( n  = 48) and Dorset ( n  = 46) sheep by PCR-RFLP and PCR-SSCP analysis. A microsatellite polymorphic site and a restriction fragment length polymorphism were shown in the 5′ regulatory region of IGF1 gene. The ewes with genotype 123/123 bp had 0.81 ( P