Detection of viral and bacterial respiratory pathogens in patients with cystic fibrosis

The presence of viral respiratory infections is associated closely with exacerbations in patients with cystic fibrosis. Viral and bacterial multiplex PCRs were developed and applied to nasal swab samples from children with cystic fibrosis. This showed a large number of individuals with cystic fibros...

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Bibliographic Details
Published in:Journal of virological methods 2012-12, Vol.186 (1-2), p.109-112
Main Authors: Stelzer-Braid, Sacha, Johal, Harpreet, Skilbeck, Kelly, Steller, Alicia, Alsubie, Haya, Tovey, Euan, Van Asperen, Peter, McKay, Karen, Rawlinson, William D.
Format: Article
Language:English
Subjects:
Adolescent
Bacteria
Bacteria - isolation & purification
Bacterial Infections - microbiology
Biological and medical sciences
Child
Child, Preschool
Children
Cystic fibrosis
Cystic Fibrosis - complications
Female
Fundamental and applied biological sciences. Psychology
Humans
Infection
Male
Microbiology
Molecular Diagnostic Techniques - methods
Multiplex PCR
Multiplex Polymerase Chain Reaction - methods
Nasal Mucosa - microbiology
Nasal Mucosa - virology
Parainfluenza
Pathogens
Polymerase chain reaction
Respiratory Tract Infections - microbiology
Respiratory Tract Infections - virology
Rhinovirus
Techniques used in virology
Virology
Virus Diseases - virology
Viruses - isolation & purification
Young Adult
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Summary:The presence of viral respiratory infections is associated closely with exacerbations in patients with cystic fibrosis. Viral and bacterial multiplex PCRs were developed and applied to nasal swab samples from children with cystic fibrosis. This showed a large number of individuals with cystic fibrosis were infected with rhinoviruses, and more were infected with viral than bacterial pathogens. All individuals with parainfluenza 3 virus had clinical exacerbations of their cystic fibrosis, and although 3/4 of these children were co-infected with HRV. The findings do not suggest a significant association for any other virus or bacteria with exacerbation. There is clear evidence some viral infections are associated with cystic fibrosis that dual infection is more likely to produce symptoms, and mechanisms of viral-induced exacerbation should be elucidated.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2012.08.008