Muscle contraction controls skeletal morphogenesis through regulation of chondrocyte convergent extension

• Published in: Developmental biology 2012-10, Vol.370 (1), p.154-163
• Main Authors: Shwartz, Yulia, Farkas, Zsuzsanna, Stern, Tomer, Aszódi, Attila, Zelzer, Elazar
• Format: Article
• Language: English
• Subjects: Alcian Blue; Animals; Biomechanical Phenomena; Bone and Bones - embryology; Cartilage; Cartilage - anatomy & histology; Cartilage - embryology; Cell Movement - physiology; Cell Shape; Chondrocyte intercalation; Chondrocytes; Chondrocytes - cytology; Chondrocytes - physiology; Convergent extension; Cytology; Danio rerio; Embryos; Evolutionary conservation; Growth plate; Growth Plate - embryology; Immunohistochemistry; In Situ Hybridization; Intercalation; Mechanical load; mechanical loads; Mechanical properties; Mice; Models, Statistical; Molecular modelling; Morphogenesis; Muscle contraction; Muscle Contraction - physiology; muscles; Neural Crest - physiology; Osteogenesis - physiology; Paralysis; Phalloidine; Pharyngeal cartilage; Pharynx; skeletal development; Skeletal muscle; Skeletogenesis; Stacking; Zebrafish
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/j.ydbio.2012.07.026

Convergent extension driven by mediolateral intercalation of chondrocytes is a key process that contributes to skeletal growth and morphogenesis. While progress has been made in deciphering the molecular mechanism that underlies this process, the involvement of mechanical load exerted by muscle contraction in its regulation has not been studied. Using the zebrafish as a model system, we found abnormal pharyngeal cartilage morphology in both chemically and genetically paralyzed embryos, demonstrating the importance of muscle contraction for zebrafish skeletal development. The shortening of skeletal elements was accompanied by prominent changes in cell morphology and organization. While in control the cells were elongated, chondrocytes in paralyzed zebrafish were smaller and exhibited a more rounded shape, confirmed by a reduction in their length-to-width ratio. The typical columnar organization of cells was affected too, as chondrocytes in various skeletal elements exhibited abnormal stacking patterns, indicating aberrant intercalation. Finally, we demonstrate impaired chondrocyte intercalation in growth plates of muscle-less Spd mouse embryos, implying the evolutionary conservation of muscle force regulation of this essential morphogenetic process.Our findings provide a new perspective on the regulatory interaction between muscle contraction and skeletal morphogenesis by uncovering the role of muscle-induced mechanical loads in regulating chondrocyte intercalation in two different vertebrate models. ► Musculature contraction is crucial for proper skeletal morphogenesis in zebrafish. ► We identify aberrant chondrocyte intercalation as a main cause for the phenotype. ► We show that this phenomenon is evolutionarily conserved by examining mutant mice.