Rapid characterization of mitochondrial genome rearrangements in Australian songbirds using next-generation sequencing technology

Using next-generation sequencing technology, we describe the complete mitochondrial genomes for 5 Australian passerine birds (Epthianura albifrons, Petroica phoenicea, Petroica goodenovii, Petroica boodang, and Eopsaltria australis). We successfully assemble each mitogenome de novo using just 1/8th...

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Bibliographic Details
Published in:The Journal of heredity 2012-11, Vol.103 (6), p.882-886
Main Authors: Cooke, Georgina M, King, Andrew G, Johnson, Rebecca N, Boles, Walter E, Major, Richard E
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Animals
Birds
Cytochromes b - genetics
Gene Order
Genetic diversity
Genome, Mitochondrial
Genomes
Genomics
Mitochondrial DNA
Molecular Sequence Data
RNA, Ribosomal
RNA, Ribosomal, 16S
RNA, Transfer
Sequence Analysis, DNA - methods
Songbirds - genetics
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Summary:Using next-generation sequencing technology, we describe the complete mitochondrial genomes for 5 Australian passerine birds (Epthianura albifrons, Petroica phoenicea, Petroica goodenovii, Petroica boodang, and Eopsaltria australis). We successfully assemble each mitogenome de novo using just 1/8th of a Roche GL FSX 454 pyrosequencing plate. From the assembled mitogenomes, we identify 2 different mitochondrial gene arrangements in the region spanning 5'-3' from Cytochrome B to 12s RNA. These gene arrangements represent 2 of the 4 known avian mitochondrial gene arrangements. Our results, together with other previously described avian mitogenomes, highlight that certain mitochondrial rearrangements appear to have arisen multiple times.
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/ess091