Alternative method for gas chromatography-mass spectrometry analysis of short-chain fatty acids in faecal samples

Short‐chain fatty acids are the major end products of bacterial metabolism in the large bowel. They derive mostly from the bacterial breakdown of carbohydrates and are known to have positive health benefits. Due to the biological relevance of these compounds it is important to develop efficient, che...

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Bibliographic Details
Published in:Journal of separation science 2012-08, Vol.35 (15), p.1906-1913
Main Authors: García-Villalba, Rocio, Giménez-Bastida, Juan A., García-Conesa, Maria T., Tomás-Barberán, Francisco A., Carlos Espín, Juan, Larrosa, Mar
Format: Article
Language:English
Subjects:
Animals
Bacteria
Biological
Biological and medical sciences
Chromatography
Faecal samples
Fatty acids
Fatty Acids, Volatile - analysis
Feces
Feces - chemistry
Gas chromatography
Gas Chromatography-Mass Spectrometry - methods
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Mass spectrometry
Mathematical analysis
Medical sciences
Metabolism
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rats
Rats, Inbred F344
Short-chain fatty acids
Solvent extraction
Spectrometry
Spectroscopy
Valeric acid
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Summary:Short‐chain fatty acids are the major end products of bacterial metabolism in the large bowel. They derive mostly from the bacterial breakdown of carbohydrates and are known to have positive health benefits. Due to the biological relevance of these compounds it is important to develop efficient, cheap, fast, and sensitive analytical methods that enable the identification and quantification of the short‐chain fatty acids in a large number of biological samples. In this study, a gas chromatography‐mass spectrometry method was developed and validated for the analysis of short‐chain fatty acids in faecal samples. These volatile compounds were extracted with ethyl acetate and 4‐methyl valeric acid was used as an internal standard. No further cleanup, concentration, and derivatization steps were needed and the extract was directly injected onto the column. Recoveries ranged between 65 and 105%, and no matrix effects were observed. The proposed method has wide linear ranges, good inter‐ and intraday variability values (below 2.6 and 5.6%, respectively) and limits of detection between 0.49 μM (0.29 μg/g) and 4.31 μM (3.8 μg/g). The applicability of this analytical method was successfully tested in faecal samples from rats and humans.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201101121