Quasi-Real Time Quantification of Uric Acid in Urine Using Boron Doped Diamond Microelectrode with in Situ Cleaning

We report herein an innovative electrochemical (EC) technique based on boron doped diamond (BDD) microelectrodes which enable the fast determination of uric acid (UA) concentrations in urine. On the basis of fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-12, Vol.84 (23), p.10207-10213
Main Authors: Kiran, Raphael, Scorsone, Emmanuel, Mailley, Pascal, Bergonzo, Philippe
Format: Article
Language:English
Subjects:
Acids
Analytical chemistry
Automation
Biosensing Techniques
Boron - chemistry
Chemistry
Diamond - chemistry
Electrocatalysis
Electrochemical methods
Electrochemistry
Electrodes
Exact sciences and technology
Humans
Microelectrodes
Spectrometric and optical methods
Uric Acid - urine
Volumetric analysis
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Summary:We report herein an innovative electrochemical (EC) technique based on boron doped diamond (BDD) microelectrodes which enable the fast determination of uric acid (UA) concentrations in urine. On the basis of fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared successfully using routine spectrophotometric diagnosis. The approach relies on the use of BDD’s superior properties such as low background current, low adsorption of species, long-term stability, and antifouling capabilities using electrochemical reactivation. Moreover, the article also describes an in situ activation technique, where the electrodes were reactivated within human urine, thereby opening the way toward automatic quantification of UA with in situ cleaning. The time taken to quantify UA concentration and cleaning remains below 0.5 s. Two analytic models were derived, based on different concentrations of ascorbic acid (AA) and uric acid, consisting of 2 s order calibration curves. Solving the second order equation enables the direct estimation of UA concentration, and values demonstrated good accuracy when compared with spectrophotometric measurements.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac301177z