Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining β-Exoglucosidases In Situ and In Vivo

A high‐end label: Cyclophellitol aziridine‐type activity‐based probes allow for ultra‐sensitive visualization of mammalian β‐glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non‐mammalian β‐glucosidases (see picture). These probes offer new ways to study β‐exoglucosidases, and configurati...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-12, Vol.51 (50), p.12529-12533
Main Authors: Kallemeijn, Wouter W., Li, Kah-Yee, Witte, Martin D., Marques, André R. A., Aten, Jan, Scheij, Saskia, Jiang, Jianbing, Willems, Lianne I., Voorn-Brouwer, Tineke M., van Roomen, Cindy P. A. A., Ottenhoff, Roelof, Boot, Rolf G., van den Elst, Hans, Walvoort, Marthe T. C., Florea, Bogdan I., Codée, Jeroen D. C., van der Marel, Gijsbert A., Aerts, Johannes M. F. G., Overkleeft, Herman S.
Format: Article
Language:English
Subjects:
activity-based probes
Animals
Aziridines - chemistry
Brain - enzymology
Cellulases - antagonists & inhibitors
Cellulases - genetics
Cellulases - metabolism
Cyclohexanols - chemistry
Cyclohexanols - metabolism
Fluorescent Dyes - chemistry
fluorescent probes
glucocerebrosidase
glycosidases
Hep G2 Cells
Humans
Isomerism
Mice
Proteomics
Recombinant Proteins - antagonists & inhibitors
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
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Summary:A high‐end label: Cyclophellitol aziridine‐type activity‐based probes allow for ultra‐sensitive visualization of mammalian β‐glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non‐mammalian β‐glucosidases (see picture). These probes offer new ways to study β‐exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity‐based probes targeting other retaining glycosidase families.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201207771