Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress

This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F(2) individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under...

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Bibliographic Details
Published in:Genetics and molecular research 2012-11, Vol.11 (4), p.3882-3888
Main Authors: Naroui Rad, M R, Abdul Kadir, M, Rafii, M Y, Jaafar, H Z E, Naghavi, M R
Format: Article
Language:English
Subjects:
Analysis of Variance
Chromosome Segregation - genetics
Crosses, Genetic
Droughts
Genetic Markers
Microsatellite Repeats - genetics
Quantitative Trait Loci - genetics
Stress, Physiological - genetics
Triticum - genetics
Triticum - physiology
Water - metabolism
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Summary:This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F(2) individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under drought stress conditions exhibited continuous variation, indicating the minor gene effects on the trait. Single-marker analysis (SMA) was carried out to detect the main QTL association with drought tolerance. The SMA results revealed that the simple sequence repeat markers GWM182 and GWM292 on chromosome 5D and GWM410 on chromosome 5A exhibited significant association with drought tolerance, accounting for 30, 22, and 21% of the total variation, respectively. The 3 genetic loci, especially GWM182, can be used in marker-assisted selection methods in drought tolerance breeding in wheat.
ISSN:1676-5680
1676-5680
DOI:10.4238/2012.November.12.5