Impact of Peptide Micropatterning on Endothelial Cell Actin Remodeling for Cell Alignment under Shear Stress

HSVEC behavior under physiological shear stress in vitro is investigated on PET surfaces micropatterned with both RGDS and WQPPRARI peptides. This technique allows (i) creating geometries on surface to guide cell orientation under shear stress and (ii) controlling surface chemical composition in ord...

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Bibliographic Details
Published in:Macromolecular bioscience 2012-12, Vol.12 (12), p.1648-1659
Main Authors: Chollet, Céline, Bareille, Reine, Rémy, Murielle, Guignandon, Alain, Bordenave, Laurence, Laroche, Gaetan, Durrieu, Marie-Christine
Format: Article
Language:English
Subjects:
Actins - physiology
adhesion
Analysis of Variance
Applied sciences
Biological and medical sciences
biomaterials
Blood Vessel Prosthesis
Cell Adhesion - physiology
Endothelial Cells - physiology
Exact sciences and technology
Fibronectins
Forms of application and semi-finished materials
Humans
In Vitro Techniques
Medical sciences
Microscopy, Fluorescence
microstructure
Oligopeptides
peptides
Polyethylene Glycols - chemistry
Polyethylene Terephthalates
Polymer industry, paints, wood
Saphenous Vein - cytology
shear
Shear Strength - physiology
Sheets and films
Surface Properties
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology of polymers
Technology. Biomaterials. Equipments
Vascular Patency - physiology
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Summary:HSVEC behavior under physiological shear stress in vitro is investigated on PET surfaces micropatterned with both RGDS and WQPPRARI peptides. This technique allows (i) creating geometries on surface to guide cell orientation under shear stress and (ii) controlling surface chemical composition in order to modulate cell behavior. Under shear stress, endothelial cells adhere on patterned PET surfaces and present a more rapid orientation in flow direction in comparison to cells cultured on homogeneous surfaces. Micropatterned surfaces presenting a large surface area ratio of RGDS/WQPPRARI peptides induce fibrillar adhesion, while surfaces presenting an equal RGDS/WQPPRARI peptides surface area ratio preferentially induce focal adhesion. Polyethylene terephthalate surfaces micropatterned with both RGDS and WQPPRARI peptides are studied in order to control endothelial cell adhesion under shear stress. These materials allow creating geometries on surface to guide cell orientation under shear stress and controlling surface chemical composition to modulate cell behavior.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.201200167