Impact of Peptide Micropatterning on Endothelial Cell Actin Remodeling for Cell Alignment under Shear Stress

• Published in: Macromolecular bioscience 2012-12, Vol.12 (12), p.1648-1659
• Main Authors: Chollet, Céline, Bareille, Reine, Rémy, Murielle, Guignandon, Alain, Bordenave, Laurence, Laroche, Gaetan, Durrieu, Marie-Christine
• Format: Article
• Language: English
• Subjects: Actins - physiology; adhesion; Analysis of Variance; Applied sciences; Biological and medical sciences; biomaterials; Blood Vessel Prosthesis; Cell Adhesion - physiology; Endothelial Cells - physiology; Exact sciences and technology; Fibronectins; Forms of application and semi-finished materials; Humans; In Vitro Techniques; Medical sciences; Microscopy, Fluorescence; microstructure; Oligopeptides; peptides; Polyethylene Glycols - chemistry; Polyethylene Terephthalates; Polymer industry, paints, wood; Saphenous Vein - cytology; shear; Shear Strength - physiology; Sheets and films; Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology of polymers; Technology. Biomaterials. Equipments; Vascular Patency - physiology
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201200167

HSVEC behavior under physiological shear stress in vitro is investigated on PET surfaces micropatterned with both RGDS and WQPPRARI peptides. This technique allows (i) creating geometries on surface to guide cell orientation under shear stress and (ii) controlling surface chemical composition in order to modulate cell behavior. Under shear stress, endothelial cells adhere on patterned PET surfaces and present a more rapid orientation in flow direction in comparison to cells cultured on homogeneous surfaces. Micropatterned surfaces presenting a large surface area ratio of RGDS/WQPPRARI peptides induce fibrillar adhesion, while surfaces presenting an equal RGDS/WQPPRARI peptides surface area ratio preferentially induce focal adhesion. Polyethylene terephthalate surfaces micropatterned with both RGDS and WQPPRARI peptides are studied in order to control endothelial cell adhesion under shear stress. These materials allow creating geometries on surface to guide cell orientation under shear stress and controlling surface chemical composition to modulate cell behavior.