Identification of quantitative trait loci for blast resistance in BC₂F₃ and BC₂F5 advanced backcross families of rice

Advanced backcross families derived from Oryza sativa cv MR219/O. rufipogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC(2)F(3) families were used to construct a linkage map, using...

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Bibliographic Details
Published in:Genetics and molecular research 2012-01, Vol.11 (3), p.3277-3289
Main Authors: Rahim, H A, Bhuiyan, M A R, Lim, L S, Sabu, K K, Saad, A, Azhar, M, Wickneswari, R
Format: Article
Language:English
Subjects:
Chromosome Mapping
Crosses, Genetic
Disease Resistance - genetics
Genetic Linkage
Inbreeding
Magnaporthe - physiology
Microsatellite Repeats - genetics
Oryza - genetics
Oryza - immunology
Oryza - microbiology
Plant Diseases - genetics
Plant Diseases - immunology
Plant Diseases - microbiology
Quantitative Trait Loci - genetics
Quantitative Trait, Heritable
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Summary:Advanced backcross families derived from Oryza sativa cv MR219/O. rufipogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC(2)F(3) families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC(2)F(3) families. Another linkage map was also constructed based on 31 BC(2)F(5) families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufipogon against M. oryzae pathotypes.
ISSN:1676-5680
1676-5680
DOI:10.4238/2012.September.12.11