The protective role of caffeic acid phenethyl ester against streptomycin ototoxicity

• Published in: American journal of otolaryngology 2013, Vol.34 (1), p.16-21
• Main Authors: Bakır, Salih, MD, Özbay, Musa, MD, Gün, Ramazan, MD, Yorgancılar, Ediz, MD, Kınış, Vefa, MD, Keleş, Ayşenur, MD, Abakay, Abdurrahman, MD, Gökalp, Osman, MD, Topçu, İsmail, MD
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Caffeic Acids - pharmacology; Disease Models, Animal; Hearing - drug effects; Hearing Loss, Sensorineural - chemically induced; Hearing Loss, Sensorineural - physiopathology; Hearing Loss, Sensorineural - prevention & control; Male; NF-kappa B - antagonists & inhibitors; Otoacoustic Emissions, Spontaneous - drug effects; Otolaryngology; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - pharmacology; Rats; Rats, Wistar; Rodents; Studies; Surgery; Treatment Outcome; Tuberculosis
• ISSN: 0196-0709; 1532-818X
• DOI: 10.1016/j.amjoto.2012.07.003

Abstract Objective The aim of this experimental study was to investigate the efficacy of caffeic acid phenethyl ester (CAPE) in the prevention of streptomycin-induced ototoxicity. Materials and Methods Thirty-two adult Wistar albino rats were divided into 4 groups: control (n = 8), streptomycin (n = 8), CAPE (n = 8), and streptomycin + CAPE (n = 8). Rats were tested with distortion product otoacoustic emissions (DPOAEs) before drug administration. The animals in all groups were killed under general anesthesia on the 45th day following last DPOAE measurements. Hearing results were analyzed statistically to determine differences in amplitudes of DPOAE. Also, the cochleas of each rat were evaluated by histopathological and immunohistochemical examination. Results Significant difference was not observed in cochlear hair cells in the control and CAPE groups. In the streptomycin group, severe degeneration of hair cells and increased apoptotic cells were observed. In the streptomycin + CAPE group, although some deteriorations were observed, hair cells were mostly preserved. The DPgram of the streptomycin and streptomycin + CAPE groups was significantly deteriorated ( P < .05). The analysis of the DPgram results revealed statistically significant differences between the groups of streptomycin and streptomycin + CAPE ( P < .05). Conclusions Caffeic acid phenethyl ester treatment attenuated hair cells injury in the inner ear, possibly via its antioxidant effect. Prophylactic administration of CAPE for streptomycin ototoxicity ameliorated hearing deterioration in rats.