Analysis of EpapGV gp37 gene reveals a close relationship between granulovirus and entomopoxvirus

The Epinotia aporema Granulovirus GP37 protein gene has been identified, located, and sequenced. This gene was similar to other baculovirus gp37, to entomopoxvirus fusolin gene, and to the chitin-binding protein gene of bacteria. Sequence analysis indicated that the open reading frame is 669 bp long...

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Bibliographic Details
Published in:Virus genes 2012-12, Vol.45 (3), p.610-613
Main Authors: Salvador, Ricardo, Ferrelli, M. Leticia, Berretta, Marcelo F, Mitsuhashi, Wataru, Biedma, Marina E, Romanowski, Víctor, Sciocco-Cap, Alicia
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
antiserum
bacteria
Base Sequence
Biomedical and Life Sciences
Biomedicine
Cydia pomonella granulovirus
Entomopoxvirinae - classification
Entomopoxvirinae - genetics
Entomopoxvirinae - immunology
Entomopoxvirinae - pathogenicity
Gene Transfer, Horizontal
genes
Genes, Viral
Glycosylation
Granulovirus - classification
Granulovirus - genetics
Granulovirus - immunology
Granulovirus - pathogenicity
Immune Sera - immunology
Lepidoptera - virology
Medical Microbiology
Open Reading Frames
Phylogeny
Plant Sciences
sequence analysis
Sequence Homology, Amino Acid
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Viral Proteins - genetics
Viral Proteins - immunology
Virology
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Summary:The Epinotia aporema Granulovirus GP37 protein gene has been identified, located, and sequenced. This gene was similar to other baculovirus gp37, to entomopoxvirus fusolin gene, and to the chitin-binding protein gene of bacteria. Sequence analysis indicated that the open reading frame is 669 bp long (the smallest gp37 sequenced at present) and encodes a predicted 222-amino acid protein. This protein is glycosylated and specifically recognized by an entomopoxvirus fusolin antiserum. The pairwise comparison of EpapGV gp37 gene product with all the baculovirus sequences in GenBank yields high similarity values ranging from 45 to 63 % with Cydia pomonella Granulovirus gp37 being the most closely related. The phylogenetic analysis interestingly grouped the granuloviruses in a cluster more closely related to entomopoxviruses than to nucleopolyhedroviruses, suggesting a possible horizontal transfer event between the granulovirus group and the entomopoxvirus group.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-012-0800-3