Inhibitory Activities of Compounds from the Twigs of Garcinia hombroniana Pierre on Human Low-density Lipoprotein (LDL) Oxidation and Platelet Aggregation

The methanol extract of the twigs of Garcinia hombroniana, which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′‐tetrahydroxy‐4‐methoxybenzophenone, 1,7‐dihydroxyxanthone and eight triterpenoids, garcihombronane B, D...

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Bibliographic Details
Published in:Phytotherapy research 2012-12, Vol.26 (12), p.1845-1850
Main Authors: Saputri, Fadlina Chany, Jantan, Ibrahim
Format: Article
Language:English
Subjects:
Adult
Aged
Antioxidants - pharmacology
antiplatelet aggregation
benzophenone
Biological and medical sciences
Garcinia - chemistry
Garcinia hombroniana Pierre (Guttiferae)
General pharmacology
Humans
LDL antioxidation
Lipoproteins, LDL - metabolism
Medical sciences
Middle Aged
Oxidation-Reduction
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - pharmacology
Platelet Aggregation - drug effects
Platelet Aggregation Inhibitors - pharmacology
xanthone
Young Adult
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Summary:The methanol extract of the twigs of Garcinia hombroniana, which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′‐tetrahydroxy‐4‐methoxybenzophenone, 1,7‐dihydroxyxanthone and eight triterpenoids, garcihombronane B, D, E and F, friedelin, glutin‐5‐en‐3β‐ol, stigmasterol and lupeol. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit copper‐mediated LDL oxidation and arachidonic acid (AA)‐, adenosine diphosphate (ADP)‐, collagen‐induced platelet aggregation in vitro. Among the compounds tested, 3,5,3′,5′‐tetrahydroxy‐4‐methoxybenzophenone and 1,7‐dihydroxyxanthone showed strong inhibitory activity on LDL oxidation with half‐maximal inhibitory concentration (IC50) values of 6.6 and 1.7 µm, respectively. 3,5,3′,5′‐Tetrahydroxy‐4‐methoxybenzophenone exhibited strong activity on AA‐, ADP‐ and collagen‐induced platelet aggregation with IC50 values of 53.6, 125.7 and 178.6 µm, respectively, while 1,7 dihydroxyxanthone showed significant and selective inhibitory activity against ADP‐induced aggregation with IC50 value of 5.7 µm. Of the triterpenoids tested, garcihombronane B showed moderate activity against LDL oxidation and garcihombronane D and F showed selective inhibition on ADP‐induced platelet aggregation. Copyright © 2012 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.4667