Inhibitory Activities of Compounds from the Twigs of Garcinia hombroniana Pierre on Human Low-density Lipoprotein (LDL) Oxidation and Platelet Aggregation

• Published in: Phytotherapy research 2012-12, Vol.26 (12), p.1845-1850
• Main Authors: Saputri, Fadlina Chany, Jantan, Ibrahim
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antioxidants - pharmacology; antiplatelet aggregation; benzophenone; Biological and medical sciences; Garcinia - chemistry; Garcinia hombroniana Pierre (Guttiferae); General pharmacology; Humans; LDL antioxidation; Lipoproteins, LDL - metabolism; Medical sciences; Middle Aged; Oxidation-Reduction; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - pharmacology; Platelet Aggregation - drug effects; Platelet Aggregation Inhibitors - pharmacology; xanthone; Young Adult
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.4667

The methanol extract of the twigs of Garcinia hombroniana, which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′‐tetrahydroxy‐4‐methoxybenzophenone, 1,7‐dihydroxyxanthone and eight triterpenoids, garcihombronane B, D, E and F, friedelin, glutin‐5‐en‐3β‐ol, stigmasterol and lupeol. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit copper‐mediated LDL oxidation and arachidonic acid (AA)‐, adenosine diphosphate (ADP)‐, collagen‐induced platelet aggregation in vitro. Among the compounds tested, 3,5,3′,5′‐tetrahydroxy‐4‐methoxybenzophenone and 1,7‐dihydroxyxanthone showed strong inhibitory activity on LDL oxidation with half‐maximal inhibitory concentration (IC50) values of 6.6 and 1.7 µm, respectively. 3,5,3′,5′‐Tetrahydroxy‐4‐methoxybenzophenone exhibited strong activity on AA‐, ADP‐ and collagen‐induced platelet aggregation with IC50 values of 53.6, 125.7 and 178.6 µm, respectively, while 1,7 dihydroxyxanthone showed significant and selective inhibitory activity against ADP‐induced aggregation with IC50 value of 5.7 µm. Of the triterpenoids tested, garcihombronane B showed moderate activity against LDL oxidation and garcihombronane D and F showed selective inhibition on ADP‐induced platelet aggregation. Copyright © 2012 John Wiley & Sons, Ltd.