Fluorescent analysis of photoinduced biodegradation of cresol isomers

• Published in: Journal of applied spectroscopy 2008-03, Vol.75 (2), p.261-267
• Main Authors: Chaikovskaya, O. N., Sokolova, I. V., Karetnikova, E. A., Maier, G. V.
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Atomic/Molecular Structure and Spectra; Biodegradation; Cresol; Cresols; Fluorescence; fluorescence spectroscopy; Fungi; Histocompatibility antigen H-2; Hydroxylation; Irradiation; Isomers; Metabolites; Mixtures; Oxidation; Penicillium; Photolysis; Physics; Physics and Astronomy; Radiation sources; Spectroscopy; U.V. radiation; Ultraviolet radiation
• ISSN: 0021-9037; 1573-8647
• DOI: 10.1007/s10812-008-9045-2

We have used fluorescence spectroscopy to study photoinduced biodegradation of ortho-cresol, para-cresol and mixture of them in water when excited by different UV radiation sources. We show that the efficiency of photolysis for both the individual isomers and the mixture of cresols is higher for excitation by radiation at λ ∼ 222 nm. Pre-irradiation of aqueous cresol solutions slows down subsequent biodegradation. Using a combination of UV irradiation and the strain Penicillium tardum H-2 for utilization of the cresol mixture led to complete degradation of para-cresol and partial degradation of ortho-cresol. Spectral and chromatographic study of the qualitative composition of the metabolites formed during biodegradation of para-cresol showed that preparatory metabolism of such compounds occurs both through oxidation of the methyl group with formation of 4-hydroxybenzoic acid and through hydroxylation with formation of 4-methylcatechol.