Albusin B modulates lipid metabolism and increases antioxidant defense in broiler chickens by a proteomic approach

• Published in: Journal of the science of food and agriculture 2013-01, Vol.93 (2), p.284-292
• Main Authors: Wang, Han-Tsung, Li, Yi-Hung, Chou, I-Pin, Hsieh, Ya-Hui, Chen, Bao-Ji, Chen, Ching-Yi
• Format: Article
• Language: English
• Subjects: albusin B; Animals; antioxidant defense; Antioxidants; Antioxidants - metabolism; ATP-Binding Cassette Transporters - antagonists & inhibitors; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; Bacteria; Bacteriocins - genetics; Bacteriocins - metabolism; broilers; Chickens - blood; Chickens - growth & development; Chickens - metabolism; Diet - veterinary; Dietary Supplements; Down-Regulation; Enzyme Induction; Fatty Acid-Binding Proteins - antagonists & inhibitors; Fatty Acid-Binding Proteins - genetics; Fatty Acid-Binding Proteins - metabolism; Female; Gene Expression Profiling - veterinary; Hydroxymethylglutaryl CoA Reductases - biosynthesis; Hydroxymethylglutaryl CoA Reductases - genetics; Hydroxymethylglutaryl CoA Reductases - metabolism; Hypolipidemic Agents - metabolism; Jejunum - enzymology; Jejunum - growth & development; Jejunum - metabolism; Lipid Metabolism; Lipids; Liver - enzymology; Liver - growth & development; Liver - metabolism; Male; Metabolism; Poultry; protein profiles; Proteins; Proteomics; Recombinant Proteins - metabolism; Superoxide Dismutase - biosynthesis; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxide Dismutase-1; Thiazoles - metabolism; Weight Gain
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.5754

BACKGROUND: The present study was designed to investigate the effect of albusin B on lipid metabolism and antioxidant defense in broiler chickens by a proteomic approach. The bacteriocin, albusin B of Ruminococcus albus 7, expressed by yeast was applied in this study. Three dietary treatments, consisting of the basal diet (control), basal diet + albusin B (2.5 g kg−1), and basal diet + nosiheptide (2.5 mg kg−1) were randomly fed to 90 broiler chickens from 1 to 35 days of age, respectively. After 35 days of supplementation, the growth performance, lipid metabolism and antioxidant proteins in the jejunum and liver, intestinal protein profile, and plasma lipid profile were analyzed. RESULTS: Broilers with albusin B supplementation had greater body weight than the control broilers. Compared with the control broilers, lower triglyceride and higher high‐density lipoprotein concentration in the blood were observed in both broilers with albusin B and nosiheptide supplementation. In addition, albusin B suppressed the mRNA expression of fatty acid binding protein 2 and ATP binding cassette transporter G 5 in the jejunum. In the jejunal protein profiles, four antioxidant proteins were upregulated by albusin B and nosiheptide treatments. The jejunal antioxidant gene expression had a concordant pattern. Hepatic genes related to lipid metabolism, 3‐hydroxy‐3‐methyl‐glutaryl CoA reductase, and superoxide dismutase were upregulated by albusin B supplementation. CONCLUSION: Albusin B supplementation modulated lipid metabolism and activated systemic antioxidant defense, which might partially contribute to the performance of broiler chickens. Copyright © 2012 Society of Chemical Industry