A proteomic analysis of the effect of growth hormone on mammary alveolar cell-T (MAC-T) cells in the presence of lactogenic hormones

• Published in: Domestic animal endocrinology 2013-01, Vol.44 (1), p.26-35
• Main Authors: Johnson, T.L., Tomanek, L., Peterson, D.G.
• Format: Article
• Language: English
• Subjects: animal ovaries; Animals; casein; cattle; Cattle - metabolism; Cell Differentiation - physiology; Cell Line; Chinese hamsters; cytoskeleton; dairy protein; desorption; dexamethasone; Dexamethasone - pharmacology; DNA; Electrophoresis, Gel, Two-Dimensional - veterinary; Epithelial Cells; Female; fibroblasts; gene expression regulation; Growth hormone; Growth Hormone - metabolism; insulin; Insulin - pharmacology; ionization; MAC-T; MALDI-TOF-TOF; Mammary; Mammary Glands, Animal - cytology; Mammary Glands, Animal - metabolism; mass spectrometry; milk; Milk Proteins - biosynthesis; oxidants; prolactin; Prolactin - pharmacology; protein folding; protein synthesis; Proteomics; Proteomics - methods; RNA; Somatotropin; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary; two-dimensional gel electrophoresis
• ISSN: 0739-7240; 1879-0054
• DOI: 10.1016/j.domaniend.2012.08.001

The bovine mammary alveolar cell-T (MAC-T) cell line is able to uniformly differentiate and secrete casein proteins in response to dexamethasone, insulin, and prolactin and is extensively used to study bovine mammary epithelial cell (MEC) function. Somatotropin, or growth hormone (GH), has been shown to increase milk protein synthesis both in vivo and in mammary cell models and to induce cytoskeletal rearrangement in a 3T3 fibroblast cell line and a Chinese hamster ovary cell line. To identify the nature of the effects of GH in MECs cultured with lactogenic hormones, changes in global protein expression were assessed in the MAC-T cell line with the use of two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization tandem time of flight mass spectrometry. Forty proteins were differentially expressed in response to GH (P < 0.05) and were related to metabolism, the cytoskeleton, protein folding, RNA and DNA processing, and oxidant stress. These widespread changes in protein expression are indicative of a global role of GH in overall cellular differentiation that may underlie the direct modulation of milk component synthesis in MEC models that have been described to date.