Metabolism of mannitol in mycorrhizal and non-mycorrhizal fungi

• Published in: The New phytologist 1987-02, Vol.105 (2), p.281-287
• Main Authors: Ramstedt, M, Jirjis, R, Soderhall, K
• Format: Article
• Language: English
• Subjects: Agaricus; Armillariella mellea; Ascomycetes; Ascomycota; Basidiomycetes; Basidiomycota; Biological and medical sciences; Carbohydrate metabolism; Dehydrogenases; ectomycorrhiza; ectomycorrhizae; Enzymes; Fundamental and applied biological sciences. Psychology; Fungi; mannitol; Mannitol metabolism; Metabolism; Metabolism. Physicochemical requirements; Mycorrhizal fungi; Phosphatases; Plant physiology and development; Sphaerosporella brunnea
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/j.1469-8137.1987.tb00865.x

The activity of mannitol dehydrogenase, mannitol 1-phosphatase and mannitol 1-phosphate dehydrogenase in five mycorrhizal basidiomycetes, one mycorrhizal ascomycete and five non-mycorrhizal basidiomycetes was investigated. No mannitol 1-phosphate dehydrogenase could be detected in any of the basidiomycetes. Mannitol dehydrogenase, present in all the tested fungi except Armillariella mellea, was strictly NAD-dependent for the non-mycorrhizal basidiomycetes, whereas in the mycorrhizal species it was mainly NADP-dependent. The mannitol 1-phosphatase of the mycorrhizal basidiomycetes was specific towards mannitol 1-phosphate in contrast to that of the non-mycorrhizal species, which was a non-specific phosphatase. In the ascomycete Sphaerosporella brunnea all the mannitol metabolizing enzymes were found, including mannitol 1-phosphate dehydrogenase. Growth of S. brunnea on mannitol slightly decreased the activity of the mannitol dehydrogenase (NADP) and mannitol 1-phosphate dehydrogenase, but there was a slight increase in activity of glucose 6-phosphate dehydrogenase.