Fresh-frozen human bone allograft in vertical ridge augmentation: clinical and tomographic evaluation of bone formation and resorption

• Published in: Cell and tissue banking 2012-12, Vol.13 (4), p.577-586
• Main Authors: Macedo, Luis Guilherme Scavone, Mazzucchelli-Cosmo, Luiz Antonio, Macedo, Nelson Luiz, Monteiro, Adriana Socorro Ferreira, Sendyk, Wilson Roberto
• Format: Article
• Language: English
• Subjects: Adult; Allografts; Alveolar Ridge Augmentation - methods; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Bone Banks; Bone density; Bone marrow, stem cells transplantation. Graft versus host reaction; Bone Resorption; Bone Transplantation - methods; Cell Biology; Clinical outcomes; Dental Implants; Female; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics; Humans; Life Sciences; Male; Marilia; Maxillofacial surgery. Dental surgery. Orthodontics; Medical sciences; Middle Aged; Osteogenesis - physiology; Skin & tissue grafts; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Teeth; Tomography, X-Ray Computed; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplant Surgery; Transplantation, Homologous; Transplants & implants
• ISSN: 1389-9333; 1573-6814
• DOI: 10.1007/s10561-011-9274-0

The aim of the current study is to evaluate fresh-frozen human bone allografts (FHBAs) used in vertical ridge augmentation clinically and by computed tomography, and to analyze the resulting bone formation and graft resorption. Sixteen FHBAs were grafted in the maxillae and mandibles of 9 patients. The FHBAs, which were provided by the Musculoskeletal Tissue Bank of Marilia Hospital (Unioss), were frozen at −80°C. After 7 months, dental implants were placed and bone parameters were evaluated. Vertical bone formation was measured by computerized tomography before (T0) and at 7 months (T1) after the surgical procedure. Bone graft resorption was measured clinically from a landmark screw head using a periodontal probe. The results were analyzed by Student’s t-test. Significant differences existed in the bone formation values at T0 and T1, with an average change of 4.03 ± 1.69 mm. Bone graft resorption values were 1.0 ± 0.82 mm (20%). Implants were placed with varying insertion torque values (35–45 Ncm), and achieved primary stability. This study demonstrates that FHBAs promote satisfactory vertical bone formation with a low resorption rates, good density, and primary implant stability.