Detection of hyaluronidase RNA and activity in urine of schistosomal and non-schistosomal bladder cancer

Diagnosis of bladder cancer is done by cystoscopy and cytology. In the last decade, many urine-based tests for bladder cancer have been developed and tested in different populations. Hence, it was relevant to assess the diagnostic significance of urinary hyaluronidase RNA and its enzyme activity in...

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Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2012-12, Vol.29 (5), p.3345-3351
Main Authors: Eissa, Sanaa, Shehata, Hanan, Mansour, Amal, Esmat, Mohamed, El-Ahmady, Omar
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Area Under Curve
Biomarkers, Tumor - urine
Bladder cancer
Female
Hematology
Humans
Hyaluronoglucosaminidase - urine
Internal Medicine
Male
Medicine
Medicine & Public Health
Middle Aged
Oncology
Original Paper
Pathology
Reverse Transcriptase Polymerase Chain Reaction
ROC Curve
Schistosomiasis haematobia - complications
Sensitivity and Specificity
Urinary Bladder Neoplasms - diagnosis
Urinary Bladder Neoplasms - enzymology
Urinary Bladder Neoplasms - urine
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Summary:Diagnosis of bladder cancer is done by cystoscopy and cytology. In the last decade, many urine-based tests for bladder cancer have been developed and tested in different populations. Hence, it was relevant to assess the diagnostic significance of urinary hyaluronidase RNA and its enzyme activity in bladder cancer. Seventy patients with bladder cancer, 56 patients with benign bladder lesions, and 49 healthy controls were enrolled in this study. Voided urine samples from all subjects were used for estimation of urinary HAase RNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and determination of its enzymatic activity by zymography. There was a significant difference in the mean ranks and positivity rates of HAase RNA expression ( P  
ISSN:1357-0560
1559-131X
DOI:10.1007/s12032-012-0295-8