Effects of Pimenta pseudocaryophyllus (Gomes) L. R. Landrum, on Melanized and Non-melanized Cryptococcus neoformans

In the present study, the in vitro susceptibility and capsular width from both melanized and non-melanized Cryptococcus neoformans cells in the presence of Pimenta pseudocaryophyllus crude extract were determined. The results were compared with those obtained for voriconazole and amphotericin B. Mel...

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Bibliographic Details
Published in:Mycopathologia (1975) 2012-12, Vol.174 (5-6), p.421-428
Main Authors: de Fátima Lisboa Fernandes, Orionalda, Costa, Carolina Rodrigues, de Souza Lino Junior, Ruy, Vinaud, Marina Clare, Hasimoto e Souza, Lúcia Kioko, de Paula, Joelma Abadia Marciano, do Rosário Rodrigues Silva, Maria
Format: Article
Language:English
Subjects:
Amphotericin B
Amphotericin B - pharmacology
Antifungal Agents - pharmacology
Antimicrobial agents
Antiparasitic agents
Biological products
Biomedical and Life Sciences
Comparative analysis
Cryptococcosis - microbiology
Cryptococcus neoformans
Cryptococcus neoformans - chemistry
Cryptococcus neoformans - drug effects
Cryptococcus neoformans - metabolism
Dopa
Drug Resistance, Fungal
Eukaryotic Microbiology
Fungal Capsules - chemistry
Fungal Capsules - metabolism
Fungi
Humans
Life Sciences
Medical Microbiology
Melanins - metabolism
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
Morphology
Mycoses
Pimenta
Pimenta - chemistry
Plant Extracts - pharmacology
Plant Sciences
Pyrimidines - pharmacology
Triazoles - pharmacology
Virulence
Voriconazole
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Summary:In the present study, the in vitro susceptibility and capsular width from both melanized and non-melanized Cryptococcus neoformans cells in the presence of Pimenta pseudocaryophyllus crude extract were determined. The results were compared with those obtained for voriconazole and amphotericin B. Melanization was obtained in minimal medium broth with the addition of L -dopa, and the antifungal susceptibility tests were performed using the broth microdilution method. Capsular width of 30 cells of each one of the isolates in medium with crude extracts of P. pseudocaryophyllus or voriconazole or amphotericin B at a concentration corresponding to 0.5 times the minimal inhibitory concentration (MIC) was measured, and the mean was calculated. The MICs and minimal fungicidal concentrations (MFCs) for plant extract and voriconazole were identical for both melanized and non-melanized C. neoformans isolates, but for amphotericin, the MFCs for melanized cells were up to 8 times higher than for non-melanized cells. The capsular width of C. neoformans cells was smaller ( p  
ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-012-9574-3