Allogeneic mRNA-based electrotransfection of autologous dendritic cells and specific antitumor effects against osteosarcoma in rats

Vaccination with dendritic cells (DCs) transfected with tumor-derived mRNA antigen has emerged as a promising strategy for generating protective immunity in mammals. However, the integration of allogeneic osteosarcoma mRNA and autologous DCs has not been fully examined. This study was designed to in...

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Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2012-12, Vol.29 (5), p.3440-3448
Main Authors: Yu, Zhe, Qian, Jixian, Wu, Jiachang, Gao, Jie, Zhang, Minghua
Format: Article
Language:English
Subjects:
Animal models
Animals
Antigen (tumor-associated)
Antigens, Neoplasm - genetics
Antigens, Neoplasm - immunology
Antitumor activity
Bone marrow
Cancer
Cancer vaccines
Cancer Vaccines - immunology
Cytotoxicity
Cytotoxicity, Immunologic
Dendritic cells
Dendritic Cells - immunology
Dendritic Cells - transplantation
Electroporation
Hematology
Immunological memory
Immunotherapy
Immunotherapy - methods
Integration
Internal Medicine
Lymphocytes T
Major histocompatibility complex
Male
Medicine
Medicine & Public Health
Monoclonal antibodies
mRNA
Oncology
Original Paper
Osteosarcoma
Osteosarcoma - genetics
Osteosarcoma - immunology
Osteosarcoma cells
Pathology
Purification
Rats
Rats, Sprague-Dawley
Rats, Wistar
RNA, Messenger - genetics
RNA, Messenger - immunology
RNA, Messenger - therapeutic use
Statistical analysis
Transfection
Transplantation, Homologous
Tumor cells
Tumors
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Summary:Vaccination with dendritic cells (DCs) transfected with tumor-derived mRNA antigen has emerged as a promising strategy for generating protective immunity in mammals. However, the integration of allogeneic osteosarcoma mRNA and autologous DCs has not been fully examined. This study was designed to investigate the antitumor effects of tumor vaccine produced by autologous DCs transfected of allogeneic osteosarcoma mRNA through electroporation in tumor-bearing rats model. In the present study, extraction of Wistar rat tumor mRNA was performed as a two-step procedure. First, total RNA was extracted by use of Trizol; then, mRNA purification was performed by use of polyT-coated magnetic beads. Then, we transfected the allogeneic-derived tumor mRNA to Sprague–Dawley (SD) rat bone marrow-derived DCs through electroporation. The tumor vaccine was applied to tumor-bearing rats model, and the specific antitumor effects of the tumor vaccine were observed. The immunization using autologous DCs electrotransfected with allogeneic osteosarcoma total RNA induced specific CTL responses, which were statistically significant ( P  
ISSN:1357-0560
1559-131X
DOI:10.1007/s12032-012-0312-y