Expression of Human Endogenous Retrovirus-K Coincides with that of Micro-RNA-663 and -638 in Germ-cell Tumor Cells

The cell line GH was established from germ-cell tumor tissue; human endogenous retrovirus-K (HERV-K) expression was detectable after prolonged culture of the cells, particularly in cells that formed domes and vesicles. In addition, keeping GH cells in culture at high cell densities increased HERV-K...

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Bibliographic Details
Published in:Anticancer research 2012-11, Vol.32 (11), p.4797-4804
Main Authors: KRAUS, Benjamin, MONK, Bettina, SLIVA, Katja, SCHNIERLE, Barbara S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Northern
Blotting, Western
Cell culture
Cell density
Endogenous Retroviruses
Gene Products, env - analysis
Gene Products, env - biosynthesis
Growth hormone
Humans
Medical sciences
MicroRNAs - biosynthesis
MicroRNAs - genetics
miRNA
Neoplasms, Germ Cell and Embryonal - genetics
Neoplasms, Germ Cell and Embryonal - virology
Prognosis
Senescence
Transcriptome
Tumor cells
Tumors
Vesicles
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Summary:The cell line GH was established from germ-cell tumor tissue; human endogenous retrovirus-K (HERV-K) expression was detectable after prolonged culture of the cells, particularly in cells that formed domes and vesicles. In addition, keeping GH cells in culture at high cell densities increased HERV-K expression. Here, we studied whether this inducible HERV-K expression is accompanied by differences in microRNA (miRNA) expression patterns of GH cells. The global miRNA expression pattern of GH cell samples (HERV-K high versus low) was analyzed by miRNA arrays. Two miRNAs were found to be differentially regulated and to exhibit expression parallel to that of HERV-K. The identified miRNAs-663 and -638, have been reported to be involved in multiple processes, including cellular senescence. However, induction of HERV-K expression did not change the cellular senescence status of GH cells. The expression of these two miRNAs might be useful as novel diagnostic and prognostic markers in patients with tumors.
ISSN:0250-7005
1791-7530