Comparative Study of Sterol Ester Synthesis using Thermomyces Lanuginosus Lipase in Stirred Tank and Packed-Bed Bioreactors

A comparative study was done on the production of different sterol esters using a stirred tank batch reactor (STBR) and packed bed reactor (PBR) using Thermomyces lanuginosus (Lipozyme TLIM) enzyme, a commercially immobilized lipase. Different oils were used as the sources of particular fatty acids,...

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Bibliographic Details
Published in:Journal of the American Oil Chemists' Society 2010-09, Vol.87 (9), p.1019-1025
Main Authors: Sengupta, Avery, Pal, Moumita, SilRoy, Sumita, Ghosh, Mahua
Format: Article
Language:English
Subjects:
Agriculture
Batch reactors
Biological and medical sciences
Biomaterials
Bioreactors
Biosynthesis
Biotechnology
Chemistry
Chemistry and Materials Science
Comparative studies
Enzymes
Esters
Fat industries
Fatty acids
Fish oils
Flow rates
Food industries
Food Science
Fundamental and applied biological sciences. Psychology
Gas chromatography
Industrial Chemistry/Chemical Engineering
Lipase‐catalyzed transesterification
Methods. Procedures. Technologies
Mustard oil
Oils & fats
Original Paper
Packed bed reactor
Phytosterol
Polyunsaturated fatty acids
Reactors
Sterol‐ester
Substrates
Thermomyces lanuginosus
Thermomyces lanuginosus (Lipozyme TLIM)
Thin layer chromatography
Various methods and equipments
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Summary:A comparative study was done on the production of different sterol esters using a stirred tank batch reactor (STBR) and packed bed reactor (PBR) using Thermomyces lanuginosus (Lipozyme TLIM) enzyme, a commercially immobilized lipase. Different oils were used as the sources of particular fatty acids, e.g., fish oil for n-3 polyunsaturated fatty acids (n-3 PUFA), linseed oil for alpha linolenic acid (ALnA) and mustard oil for erucic acid. Reaction parameters, such as substrate molar ratio, reaction temperature and enzyme concentration, were standardized in the STBR and maintained in the PBR. To provide equal time of residence between the substrate and enzyme in both the reactors for the same amount of substrates, the substrate flow rate in the PBR was maintained at 0.27 ml/min. Thin layer chromatography was used to monitor the reaction, and column chromatography was used to determine the product yields. Fatty acid compositions of the esters were determined by gas chromatography. The study showed that the packed bed bioreactor was more efficient than the batch reactor in sterol-ester synthesis with less migration of acyl groups.
ISSN:0003-021X
1558-9331
DOI:10.1007/s11746-010-1587-9