Preparative isolation and purification of harpagoside and angroside C from the root of Scrophularia ningpoensis Hemsley by high-speed counter-current chromatography

In this study, the bioactive component harpagoside and angroside C in the root of Scrophularia ningpoensis Hemsley was simultaneously separated by high‐speed counter‐current chromatography (HSCCC). A two‐phase solvent system containing chloroform/n‐butanol/methanol/water (4:1:3:2, v/v/v/v) was selec...

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Bibliographic Details
Published in:Journal of separation science 2012-10, Vol.35 (19), p.2659-2664
Main Authors: Tian, Jinfeng, Ye, Xiaoli, Shang, Yuanhong, Deng, Yafei, He, Kai, Li, Xuegang
Format: Article
Language:English
Subjects:
Angroside C
Biological and medical sciences
Chloroform
Chromatography
Countercurrent Distribution - methods
General pharmacology
Glycosides - analysis
Glycosides - isolation & purification
Harpagoside
Herbal medicine
High speed
High-speed counter-current hromatography
Medical sciences
Methyl alcohol
Partitions
Pharmacognosy. Homeopathy. Health food
Pharmacology
Pharmacology. Drug treatments
Plant Extracts - analysis
Plant Extracts - isolation & purification
Plant Roots - chemistry
Purification
Pyrans - analysis
Pyrans - isolation & purification
Roots
Scrophularia - chemistry
Scrophularia ningpoensis
Solvents
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Summary:In this study, the bioactive component harpagoside and angroside C in the root of Scrophularia ningpoensis Hemsley was simultaneously separated by high‐speed counter‐current chromatography (HSCCC). A two‐phase solvent system containing chloroform/n‐butanol/methanol/water (4:1:3:2, v/v/v/v) was selected following consideration of the partition coefficient of the target compound. The crude extract (200 mg) was loaded onto a 280‐mL HSCCC column and yielded 22 mg harpagoside and 31 mg angroside C with the purity of higher than 98 and 98.5%, respectively. It is feasible to isolate active compounds harpagoside and angroside C from S. ningpoensis using HSCCC.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201200337