Aqueous phase size exclusion chromatography of polyvinylalcohols of different degrees of hydrolysis

• Published in: Macromolecular symposia. 2003-07, Vol.197 (1), p.101-114
• Main Authors: Lacík, Igor, Šnauková, Radka, Šimková, Barbora, Hanzelová, Silvia, Alexy, Pavol
• Format: Article
• Language: English
• Subjects: aqueous phase SEC; degree of hydrolysis; Extrusion; Fluid dynamics; hydrodynamic equivalence ratio; Hydrolysis; Molecular weight; Molecular weight distribution; Polyvinyl alcohols; polyvinylalcohol; Size exclusion chromatography; Suprema columns
• ISSN: 1022-1360; 1521-3900
• DOI: 10.1002/masy.200350710

The aqueous‐phase size exclusion chromatography (SEC) represents a suitable method for the analysis of molecular weight distribution (MWD) of polyvinylalcohols (PVOHs) of different degree of hydrolysis ranging from 72 to 98 mol‐% applying the Suprema columns. Eluents of different composition were used consisting of either a mixture of water and acetonitrile for the lower degrees of hydrolysis (72 ‐ 88 mol‐%) or 0.1 M LiNO3 solution for the higher degrees of hydrolysis (88 ‐ 98 mol‐%). By knowing the M̄w̄ values of commercially available PVOHs samples of a given degree of hydrolysis and using the commercial water soluble narrow distribution standards, a principle of the constant hydrodynamic equivalence ratios was applied to obtain the absolute values of the molecular weights of PVOHs of different degree of hydrolysis. The effect of a various number of extrusion cycles on MWD of different PVOHs was used to demonstrate the sensitivity of the selected SEC conditions.