Evaluation of paracetamol distribution and stability in case of acute intoxication in rats

• Published in: Human & experimental toxicology 2013-01, Vol.32 (1), p.82-89
• Main Authors: Battal, D, Hilal, A, Daglioglu, K, Unal, I, Gulmen, MK
• Format: Article
• Language: English
• Subjects: Acetaminophen - blood; Acetaminophen - pharmacokinetics; Acetaminophen - toxicity; Acute intoxication; Analgesics; Analgesics, Non-Narcotic - blood; Analgesics, Non-Narcotic - pharmacokinetics; Analgesics, Non-Narcotic - toxicity; Animals; Biological and medical sciences; Brain - metabolism; Drug intoxications. Doping; Kidney - metabolism; Laboratory animals; Liver - metabolism; Male; Medical sciences; Pharmacology; Pharmacology. Drug treatments; Rats; Rats, Wistar; Specimen Handling; Tissue Distribution; Toxicity
• ISSN: 0960-3271; 1477-0903
• DOI: 10.1177/0960327112456314

Effects of different storing conditions on paracetamol concentration in biological samples of acute intoxicated rats were investigated. The stability and distribution of paracetamol was observed in postmortem serum, liver, kidney and brain tissues. The serum samples were stored for 30 days and daily changes were evaluated for paracetamol. A significant difference (p = 0.05) was noticed on the 30th experimental day. Paracetamol serum levels changed as much as 66.30% and 33.78% for 4°C and −20°C, respectively. The stability of paracetamol in liver stored at −20°C was also evaluated for 30 days. The paracetamol concentration levels taken from liver samples dramatically decreased from 30.36% on the 1st day to 94.97% on the 30th day. The paracetamol distribution in organs was as 2.68 , 1.11 and 0.68 mg/g in liver, kidney and brain samples, respectively. Meaningful difference in paracetamol in serum and liver samples was in observed in 30th day values (p = 0.05).