Angiopoietin-2/Tie2 signaling involved in TNF-a induced peritoneal angiogenesis

• Published in: International journal of artificial organs 2012-09, Vol.35 (9), p.655-662
• Main Authors: Yuan, Jiangzi, Fang, Wei, Lin, Aiwu, Ni, Zhaohui, Qian, Jiaqi
• Format: Article
• Language: English
• ISSN: 0391-3988
• DOI: 10.5301/ijao.5000123

Purpose: Angiopoietin-2/Tie2 signaling has been found to play an important role in producing a vasculature in a variety of conditions. However, whether angiopoietin-2/Tie2 signaling is involved in peritoneal angiogenesis induced by TNF-a is not clear. In this study, we investigated the role of TNF-a on the function of human omental tissue microvascular endothelial cells (HOTMECs) and whether angiogenesis is inhibited by blocking angiopoietin-2/Tie2 signaling.?Methods: Primary cultured HOTMECs were exposed to complete medium as control, medium containing 10 ng/ml TNF-a, a mixture of 10 ng/ml TNF-a plus 2 mu g/ml sTie2/Fc or 2 mu g/ml sTie2/Fc alone, respectively, as experimental groups. The proliferation of HOTMECs was measured by MTT assay. Expression of vascular endothelial growth factor (VEGF), Angiopoietin-2, and Tie2 were assessed by real-time PCR. We also investigated the angiogenesis of the HOTMECs by tube formation assay, their migration as well as their permeability to FTTC-labeled BSA.?Results: Compared to the cells in control, exposure to TNF-a or sTie2/Fc had no effect on proliferation of HOTMECs. TNF-a up-regulated the gene expression of VEGF, Angiopoietin-2, and Tie2 (p